61911

Atto 520-Azid

suitable for fluorescence

• UNSPSC Code: 12352200

Eigenschaften

• assay: ≥80.0% (HPCE)
• form: solid
• mol wt: M_w 681 g/mol
• manufacturer/tradename: ATTO-TEC GmbH
• fluorescence: λ_ex 516 nm; λ_em 545 nm±10 nm in PBS, pH 7.4
• suitability: suitable for fluorescence
• storage temp.: −20°C

Beschreibung

General description

Atto 520 is a novel fluorescent label related to the well-known dye Rhodamine 6G. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. The dye is moderately hydrophilic. Above pH 7.0 the dye exists in an equilibrium with a colourless form (pseudobase). This is a reversible reaction, which does not interfere with the process of coupling to proteins etc. On lowering the pH absorption and fluorescence are restored fully. For details of coupling see our recommended labeling procedures The fluorescence is excited most efficiently in the range 510 - 535 nm. A suitable source of excitation is an Argon-Ion laser using the 514 nm line.
The azide modification is suitable for reactions with alkyne groups (Huisgen reaction - “Click Chemistry“).

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Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable