Here's the recommended Coating Procedure:
Add 5 ml of sterile 0.01 N HCl solution to the VitroCol® serum vial containing 15 mg.
Gently mix contents until material is completely solubilized. It may be necessary to agitate at 2 to 10°C overnight.
Transfer the desired volume of solution from the serum vial to a dilution vessel if required. Further dilute to the desired concentration using sterile 0.01 N HCl solution. A typical working concentration may range from 50 to 100 ug/ml.
Note: Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.
Add an appropriate amount of diluted VitroCol® material to the culture surface.
Incubate at room temperature, covered, for 1-2 hours. Aspirate any remaining material. Alternatively, incubate at room temperature until the surface is dry.
After incubation, aspirate any remaining material.
Rinse coated surfaces carefully with sterile medium or PBS, avoiding scratching surfaces.
Coated surfaces are ready for use. They may also be stored at 2-10°C, damp or air dried if sterility is maintained.
5008
Human Collagen Type I & III
from neo-natal fibroblast cells, 97% Type I with the remainder of Type III collagen, powder, suitable for cell culture, VitroCol®
Synonym(e):
Collagen Solution
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Über diesen Artikel
UNSPSC Code:
12352202
NACRES:
NA.77
EC Number:
232-697-4
Biological source:
human
Form:
lyophilized
Technique(s):
cell culture | mammalian: suitable
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Unterstützung erhaltenProduktname
VitroCol®, Collagen from Human neonatal fibroblasts, 97% Type I with the remainder of Type III collagen, suitable for cell culture
biological source
human
Quality Level
sterility
sterile-filtered
form
lyophilized
packaging
pkg of 15 mg
manufacturer/tradename
Advanced BioMatrix
technique(s)
cell culture | mammalian: suitable
impurities
<1 EU/mL Endotoxin level
shipped in
wet ice
storage temp.
−20°C
General description
VitroCol® collagen is naturally secreted from human neo-natal fibroblast cells. The human fibroblasts are cultured in optimal conditions allowing the fibroblasts to naturally and efficiently secret extracellular matrix. The extracellular matrix is then processed and purified to yield the naturally produced human collagen.
VitroCol® is approximately 97% Type I human collagen with the remainder being comprised of Type III collagen. It contains high monomer content as measured by gel permeation chromatography.
VitroCol® is especially ideal for human cell culture systems when coating of surfaces and providing preparations of thin layers of culturing cells. It is not recommend for the formation of a solid gel. VitroCol® is supplied as a sterile, lyophilized powder.
VitroCol® is approximately 97% Type I human collagen with the remainder being comprised of Type III collagen. It contains high monomer content as measured by gel permeation chromatography.
VitroCol® is especially ideal for human cell culture systems when coating of surfaces and providing preparations of thin layers of culturing cells. It is not recommend for the formation of a solid gel. VitroCol® is supplied as a sterile, lyophilized powder.
Preparation Note
This product is supplied as a lyophilized powder with 15 mg of human collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.
Legal Information
VitroCol is a registered trademark of Advanced BioMatrix, Inc.
Lagerklasse
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 5008-15MG | 04061834430544 |
| HLMIR1962 | 04061836727703 |
| HLTUD1962 | 04061836096298 |
| HLTUD1961 | 04061836096281 |
| HLMIR1961 | 04061836727697 |
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Which is the working concentration if I intend to use it for flask coating?
1 answer-
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