250-05N

Human Umbilical Vein Smooth Muscle Cells: HUVSMC, neonatal

• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: human umbilicus (normal veins)
• Growth mode: Adherent
• Morphology: smooth muscle
• Relevant disease(s): cardiovascular diseases

Properties

• biological source: human umbilicus (normal veins)
• Quality Segment: 100
• packaging: pkg of 500,000 cells
• manufacturer/tradename: Cell Applications, Inc
• growth mode: Adherent
• karyotype: 2n = 46
• morphology: smooth muscle
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cardiovascular diseases
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

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HUVSMC has been used in studies evaluating treatments that involve blood vessel stents. Specifically, intra-shunt delivery of motexafin gadolinium was shown to inhibit the proliferation of SMCs during treatment of portal hypertension (Wang, 2013). Also, high-density lipoproteins (HDL) exhibit cardioprotective and anti-inflammatory properties by reducing HUVSMC expression of chemokines CCL2, CCL5, and CX3CL1, and chemokine receptors CCR2 and CX3CR1 (via NF-κB/pAkt inhibition) and by decreasing cell proliferation (via pERK inhibition), highlighting HDL importance in preventing neointimal hyperplasia, the main cause of early vein graft/stent failure (van der Vorst, 2013). HUVSMC were also used to demonstrate that genes coding for muscle and non-muscle actin proteins are transcribed by different transcription machinery (Lee, 2011).Characterization: positive for smooth muscle cell specific alpha-actin expression.

Application

cell adhesion, pharmacology, vascular therapy research, coagulation, drug discovery, regulation of vascular tone

Biochem/physiol Actions

Vein

Preparation Note

• 2nd passage, >500,000 cells in Smooth Muscle Cell Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 6 doublings

Please refer to the HUVSMC Culture Protocol.

Other Notes

Smooth Muscle Cell Basal Medium containing 10% FBS & 10% DMSO

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable