No direct reconstitution protocol is available for this product. Based on an online literature search, the CFSE dye, purchased as carboxyfluorescein diacetate succinimidyl ester (MW 557.47 g/mole), is typically provided in 25 mg vials. To prepare a final stock solution of 5 mM, dissolve the 25 mg in 8.96 mL DMSO. This solution can be stored as 50-100 μL aliquots at -20 °C for several months. It is important to avoid exposure to aqueous solutions during storage. Note that the CFSE dye is at a final concentration of 5 μM, however, the optimum concentration may have to be determined for each batch prepared, and then checked every 6 months to ensure it is effective.
Fortfahren mit
21888
5(6)-Carboxyfluoreszeindiacetat-N-succinimidylester
BioReagent, suitable for fluorescence, ≥90% (HPLC)
Synonym(e):
CFSE
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
25 mg | Warenkorb auf Verfügbarkeit prüfen | € 461,00 |
100 mg | Warenkorb auf Verfügbarkeit prüfen | € 1.260,00 |
Über diesen Artikel
Empirische Formel (Hill-System):
C29H19NO11
CAS-Nummer:
Molekulargewicht:
557.46
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352108
MDL number:
Technischer Dienst
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Unterstützung erhaltendescription
suitable as fluorogenic substrate for esterases
Quality Segment
product line
BioReagent
assay
≥90% (HPLC)
form
solid
mol wt
557.46 g/mol
color
almost white to yellow
mp
152-154 °C (lit.)
solubility
DMF: soluble, DMSO: soluble
fluorescence
λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)
suitability
suitable for fluorescence
storage temp.
−20°C
SMILES string
CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(=O)ON6C(=O)CCC6=O)c1.CC(=O)Oc7ccc8c(Oc9cc(OC(C)=O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)ON%12C(=O)CCC%12=O)c7
InChI
1S/2C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34;1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h2*3-8,11-13H,9-10H2,1-2H3
InChI key
LZFDYQHOQDFMOA-UHFFFAOYSA-N
General description
5-(und 6-)Carboxyfluoresceindiacetat-N-Succinimidylester (CFSE) ist ein fluoreszierender Farbstoff, der membrandurchlässig ist. Er markiert Zellen, indem er kovalent an intrazelluläre Moleküle bindet. CFSE wird zur Verfolgung der Migration und Proliferation von Lymphozyten eingesetzt.
Application
5-(6-)Carboxyfluoresceindiacetat-N-Succinimidylester (CFSE) ist eine Amin-reaktive fluoreszierende Sonde, die zur Färbung von Zellen, insbesondere Immunzellen, für Analysen in Anwendungen wie der Durchflusszytometrie verwendet wird.[1][2] Bildet bei Deacetylierung grün fluoreszierende Konjugate.
Amin-reaktives Label für die Langzeit-Überwachung von Zellen. Bildet nach Deacetylierung grün fluoreszierende Konjugate.
Verwendung von 5-Carboxyfluoresceindiacetat-N-Succinimidylester:
- zur Markierung von Zellen für die Durchflusszytometrie
- bei Proliferationsassays von Lymphozyten
- zur Bestimmung des intrazellulären oder intraorganellen pH-Werts in Chloroplasten
- zur Markierung von Leishmania amazonensis und Leishmania major
Biochem/physiol Actions
5-(6-)Carboxyfluoresceindiacetat-N-Succinimidylester wird als Indikator für die Esterase-Aktivität verwendet.
Packaging
Bodenlose Glasflasche. Der Inhalt befindet sich innerhalb des eingeschobenen Schmelzkegels.
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Dieser Artikel | |||
|---|---|---|---|
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence |
| fluorescence λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase) | fluorescence λex 470 nm; λem 529 nm in 0.1 M Tris pH 8.0 (esterase) | fluorescence λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase) | fluorescence λex 492 nm; λem 517 nm in DMF |
| color almost white to yellow | color - | color - | color - |
| solubility DMF: soluble, DMSO: soluble | solubility DMF: soluble, DMSO: soluble, methanol: soluble | solubility DMSO: soluble | solubility DMF: soluble, DMSO: soluble |
| form solid | form - | form powder | form powder |
| assay ≥90% (HPLC) | assay ≥85% (HPCE) | assay ≥90.0% (HPLC) | assay ≥80% (HPLC) |
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Hi. I bought your CFSE. I want to ask how to use it? I follow one paper: doi: 10.1016/j.xcrm.2025.102027. They used CFSE in the final concentration of 0.5 uM for 3 mins at RT. Is this you suggested? And if yes, how to prepare 0.5 uM?
1 answer-
Helpful?
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What is the in vivo (mouse) half-life of CFDA-SE? What is the route of clearance (kidney, liver?)
1 answer-
The half-life is not determined for this product.
Please see the link below to a publication that may be helpful:
https://www.semanticscholar.org/paper/The-Use-of-Carboxyfluorescein-Diacetate-Ester-to-Quah-Parish/cae3435e063cb9f46cea4ddfc7c560f5821e19b3Please note that, unless otherwise stated, this product is intended for research use only and is not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Helpful?
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What is the ratio between the (5) and (6) isomer of Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester?
1 answer-
Our Quality Control group does not routinely report the ratio; however, it is very likely that both isomers are present.
Helpful?
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At what concentration is Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, soluble in DMSO?
1 answer-
We have found 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester to be soluble in DMSO at a concentration of 1 mg/mL.
Helpful?
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Is Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, light sensitive?
1 answer-
Yes. The powder and solutions (especially solutions) should be kept protected from ambient light whenever possible.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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Is Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, fluorescent? Are conjugates of this compound fluorescent?
1 answer-
No. This compound and conjugates of this compound become fluorescent only when acted upon by an enzyme such as esterase. The enzyme removes the acetate groups.
Helpful?
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