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Merck

17389

Sigma-Aldrich

Pektinase aus Aspergillus niger

powder, slightly beige, >1 U/mg

Synonym(e):

Polygalacturonase

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About This Item

CAS-Nummer:
EC-Nummer:
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352204
NACRES:
NA.54

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Biologische Quelle

Aspergillus niger

Form

powder

Spezifische Aktivität

>1 U/mg

Farbe

slightly beige

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Pectinase, also known as pectinolytic enzyme, is predominantly found in bacteria, fungi and plants.[1] polygalacturonase is a highly found enzyme among all the pectinolytic enzymes.[1]

Anwendung

Pectinase from Aspergillus niger has been used to hydrolyze pectin layers in
  • activated sludge flocs[2]
  • bamboo powder for degumming process[3]
  • garden cress (Lepidium sativum) seeds to enhance testa permeability, visualized using tetrazolium staining solution[4]

Biochem./physiol. Wirkung

Pectinase found in plants aids in cell wall extension and softening of some plant tissues during maturation and storage. Polygalacturonase is the enzyme that catalyzes the hydrolysis of the polygalacturonic acid chain by addition of water. [1] Pectinase has wide range of industrial applications including, fruit juice extraction and its clarification[1][2], scouring of cotton, degumming of plant fibers and waste water treatment. In addition, it is also used in vegetable oil extraction, tea and coffee fermentations, bleaching of paper, in poultry feed additives and in alcoholic beverages and food industries.[1]

Einheitendefinition

1 U entspricht der Enzymmenge, die 1 μmol Galacturonsäure aus Polygalacturonsäure pro Minute bei pH 4.0 und 50°C freisetzt

Piktogramme

Health hazard

Signalwort

Danger

H-Sätze

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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Die Dokumentenbibliothek aufrufen

Microbial pectinolytic enzymes: a review
Jayani RS, et al.
Process Biochemistry (Oxford, United Kingdom), 40(9), 2931-2944 (2005)
Aránzazu Manzano et al.
American journal of botany, 108(9), 1775-1792 (2021-09-16)
Light and gravity are fundamental cues for plant development. Our understanding of the effects of light stimuli on plants in space, without gravity, is key to providing conditions for plants to acclimate to the environment. Here we tested the hypothesis
Bamboo fibre processing: insights into hemicellulase and cellulase substrate accessibility
Fu Jiajia, et al.
Biocatalysis and Biotransformation, 30(1), 27-37 (2012)
Eman Ibrahim et al.
Journal of biochemistry, 169(1), 109-117 (2020-08-19)
Lignocellulosic biomass conversion using cellulases/polygalacturonases is a process that can be progressively influenced by several determinants involved in cellulose microfibril degradation. This article focuses on the kinetics and thermodynamics of thermal inactivation of recombinant Escherichia coli cellulases, cel12B, cel8C and
Enzyme disintegration with spatial resolution reveals different distributions of sludge extracellular polymer substances
Lu Fan, et al.
Biotechnology for Biofuels, 9(1), 29-29 (2016)

Questions

  1. Pectinase from Aspergillus powder reference 17389 Hi, can you suggest a publication with data of enzyme activity as a function of pH and temperature? Thank you in advance for your help.

    1 answer
    1. Specific articles on pH and temperature range citing this product are not currently available. Please see the link below to review a publication that may be helpful:

      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4564381/#:~:text=The%20partially%20purified%20pectinase%20from,thermostable%20and%20alkaline%20in%20nature.

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