10072802
CCB cell line from carp
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Alle Fotos(1)
About This Item
UNSPSC-Code:
41106514
NACRES:
NA.81
Empfohlene Produkte
Biologische Quelle
fish brain
Wachstumsmodus
Adherent
Karyotyp
Not specified
Morphologie
Squamous
Produkte
Not specified
Rezeptoren
Not specified
Methode(n)
cell culture | mammalian: suitable
Versandbedingung
dry ice
Lagertemp.
−196°C
Ursprung der Zelllinie
Common carp brain cell line, koi herpesvirus isolation
Beschreibung der Zelllinie
The CCB cell line was developed from Common carp (Cyprinus carpio carpio) brain.
Nährmedium
EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). 10 mM HEPES can be included if a CO2 incubator is not available.
Subkultur-Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:6, i.e., seeding at 2 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 25 °C.
Sonstige Hinweise
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Irene Cano et al.
International journal of molecular sciences, 21(22) (2020-11-15)
Seroconversion and the mucosal lysozyme G (lysG), complement 3 (c3), and immunoglobulins M (IgMsec) and Z2 (IgZ2) were measured for up to 900 degree days (DD) in skin swabs from common carp exposed to koi herpesvirus (KHV or CyHV-3) at
Irene Cano et al.
Animals : an open access journal from MDPI, 11(2) (2021-02-13)
Fluorescence real-time LAMP assays were designed for the orf43 gene of CyHV-3 European genotype and the p4a gene of the CEV genogroup I. A third LAMP assay to detect the ef1a gene of the host common carp was designed as
Agnieszka Troszok et al.
Journal of fish diseases, 41(11), 1709-1718 (2018-08-26)
Cyprinid herpesvirus 3 (CyHV-3), also known as koi herpesvirus (KHV), is an aetiological agent of a virulent and lethal disease in common and koi carp. In this study, we examined in vitro the anti-CyHV-3 activity of acyclovir (ACV), nucleoside analogue commonly
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