Catalog number 08062601 is from the organization called ECACC. Please contact ECACC directly for detailed, and questions about passage experience with this cell line. https://www.culturecollections.org.uk/about-us/ecacc/
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Über diesen Artikel
NACRES:
NA.81
UNSPSC Code:
41106514
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
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Unterstützung erhaltenQuality Segment
form
liquid
packaging
tube of 5 μg 08062601-DNA-5UG, pkg of vial of cells 08062601-1VL
General description
For complete product information, please see ECACC
Application
Recommended culture medium: DMEM + 2mM Glutamine + 10% FBS
Biochem/physiol Actions
A somatic cell hybrid of a rat embryonic dorsal root ganglion (DRG) and mouse neuroblastoma cell line N18TG2. The line retains both rat and mouse chromosomes and synthesises both rat and mouse isoenzymes. Manifests a unique combination of action potential properties and cell surface markers.
Packaging
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Lagerklasse
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Dear all, I have two question for you about F-11 cells: -) When thawing for the first time, is DMEM+ 2mM Glutamine+ FBS 10% medium used? -) How many passages can be made for these cells? Thank you in advance.
1 answer-
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Are there specific requirements for the DMEM variation needed for culturing cells if the cells do not appear healthy after thawing and plating for the first time? I purchased D6429 (Sigma) for this purpose, but the next day, most cells were floating and the ones that were attached did not look 100% healthy. I'm uncertain if it's due to this batch of cells or if the media is off.
1 answer-
Please consider using DMEM from cat#D6546, recommended by the European Collection of Authenticated Cell Cultures (ECACC). This product has a different formulation from D6429 as it contains L-Glutamine in addition to the 2mM Glutamine + 10% FBS recommended to add to DMEM. For more information on the composition of D6546 and D6429, please refer to the following web page: Composition of D6546 and D6429. If using D6429 instead of D6546, it is advisable to consider adding more L-Glutamine as recommended by ECACC. For a list of product numbers, please consult page 66 of 67 in the ECACC lab handbook, available at the following link: ECACC Lab Handbook.
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