This product is a 1 mg/mL solution of BSA in 0.15 M NaCl. There are no anticipated problems with further diluting in Tris-based or other aqueous buffer solutions for blocking purposes.
P0914
Protein-Standard
Micro Standard, liquid
Synonym(e):
Albumin aus Rinderserum, Proteinstandard
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5 AMP
€ 82,90
10 AMP
€ 147,00
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5 AMP
€ 82,90
10 AMP
€ 147,00
About This Item
Empfohlene Produkte
Biologische Quelle
bovine
Qualitätsniveau
Form
liquid
Enthält
0.05% sodium azide as preservative
Verpackung
ampule of 1 mL
Konzentration
0.98—1.02 mg protein/mL
Methode(n)
ELISA: suitable
Farbe
clear colorless
UniProt-Hinterlegungsnummer
Lagertemp.
2-8°C
Angaben zum Gen
bovine ... ALB(280717)
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Anwendung
Der Proteinstandard wird wie folgt verwendet:
Proteinstandard für alle Proteinassays Die versiegelten Ampullen gewährleisten, dass die Konzentration in jedem Assay stimmt.
Proteinstandard für alle Proteinassays Die versiegelten Ampullen gewährleisten, dass die Konzentration in jedem Assay stimmt.
Konzentrierte Lösung für alle Protein-Assays.
Verpackung
verschweisste Ampullen
Sonstige Hinweise
1 mg Rinderserumalbumin/ml in 0.15 M NaCl
Eignung
Dieses Produkt wird üblicherweise für die Protein-Bestimmung nach der Bicinchoninsäure (BCA)-Methode verwendet.
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
nwg
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Analysenzertifikate (COA)
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Protokolle
To standardize a procedure for the determination of protein by modified Lowry.
To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.
Verwandter Inhalt
Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.
Proteinquantifizierungsmethoden, Reagenzien und Immunassaytechnologie für die präzise Messung der Proteinkonzentration in unterschiedlichen Proben.
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Can bsa P0914-5AMP be diluted in tbs-t to make block buffer for electrophoresis?
1 answer-
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What is the shipping temperature?
1 answer-
The item is shipped at ambient temperature. A transit time of up to 2 weeks will not impact the quality of this material. Upon delivery, this product should be stored at 2-8°C.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Do you have any information on the traceability to a recognized protein standard, such as NIST? Or is the concentration derived from accepted values of natural physical constants?
1 answer-
The concentration of each lot of the protein standard, Product P0914, is determined by UV measurements. Specifically, given a known extinction coefficient (E) and a measured absorbance (A), and assuming a standard pathlength of 1 cm, the concentration (c) can be determined by Beer's Law (c = A/E). The assay result is derived from an accepted value of a natural physical constant. Specifically, the E1% of 6.67 at approximately 279 nm is a well-accepted value, originally published in J. Am. Chem. Soc., 78, 3656-3660 (1956).
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