93313

Trizma^®-hydrochlorid-Pufferlösung

BioUltra, Molecular Biology, pH 7.4

• Synonym(e): Tris-(hydroxymethyl)-aminomethan-hydrochlorid-Pufferlösung
• NACRES: ND.02
• PubChem Substance ID: 329770223
• eCl@ss: 32129211
• UNSPSC Code: 12352200
• MDL number: MFCD00012590
• Beilstein/REAXYS Number: 3675235

Eigenschaften

• grade: Molecular Biology
• Quality Segment: 100
• product line: BioUltra
• form: liquid
• concentration: 1.0 M tris-HCl
• impurities: DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected
• pH: 7.4±0.1 (25 °C)
• density: 1.05 g/mL at 20 °C
• cation traces: Al: ≤1 mg/kg, As: ≤0.1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Na: ≤20 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg
• λ: neat
• UV absorption: λ: 260 nm A_max: 0.03, λ: 280 nm A_max: 0.04
• SMILES string: Cl.NC(CO)(CO)CO
• InChI: 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
• InChI key: QKNYBSVHEMOAJP-UHFFFAOYSA-N

Beschreibung

Application

• Reduced glutathione inhibits rabbit and rat skeletal muscle lactate dehydrogenase and prevents dinitrophenol induced extracellular acidification by an epithelial cell line.: This study investigates how Trizma^® hydrochloride buffer solution maintains pH stability, critical for accurately assessing the biochemical interactions in muscle and epithelial cells (Wimberger et al., 1997).

Other Notes

Buffer component (SET buffer) in the preparation of RNA.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves