861006

Eosinscharlach

certified by the BSC, Dye content 90 %

• Synonym(e): 4′,5′-Dibrom-2′,7′-dinitro-fluorescein Dinatriumsalz, Eosin B, Eosin bläulich, Saffrosin, Säurerot 91
• Lineare Formel: C₂₀H₆N₂O₉Br₂Na₂
• CAS-Nummer: 548-24-3
• Molekulargewicht: 624.06
• NACRES: NA.47
• PubChem Substance ID: 24888525
• UNSPSC Code: 12171500
• EC Number: 208-943-1
• MDL number: MFCD00005041
• Beilstein/REAXYS Number: 4121964
• Colour Index Number: 45400

Eigenschaften

• agency: certified by the BSC
• Quality Segment: 200
• form: powder
• composition: Dye content, 90%
• technique(s): microbe id | staining: suitable
• solubility: H₂O: 1 mg/mL, clear, red
• λmax: 395 nm, 514 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(C(=O)C(Br)=C3Oc4c(Br)c([O-])c(cc24)[N+]([O-])=O)[N+]([O-])=O
• InChI: 1S/C20H8Br2N2O9.2Na/c21-14-16(25)11(23(29)30)5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(24(31)32)17(26)15(22)19(10)33-18(9)14;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2
• InChI key: GYYTYUGGVBYJHE-UHFFFAOYSA-L

Beschreibung

Application

Gegenfärbung für Kollagen, in der Mayer Hämalaun-Methode. Verwendet mit Azur A als Gewebefarbstoff für Zell-Granula, Kerne und Mikroorganismen. Für Differential-Färbung von Zellen der vorderen Hypophyse.

• Eosin B has been used for the histological analysis of anal sphincter sections obtained from rats.
• It has been used along with Mayer′s hematoxylin for the staining of triangularis sterni muscle sections.
• It has been used along with hematoxylin for the staining of different tissue sections obtained from mouse.

Biochem/physiol Actions

Eosin B is a xanthene dye and is also referred to as eosin bluish or erythrosin B. It is used in the Harris stain for Negri bodies. Eosin B is also used in Mayer′s Hemalum as a counterstain for collagen. Eosin B-Azure A are used together for the staining of cell granules, nuclei and microorganisms. It can also be used for protein estimation. The protein - eosin B complex absorbs at wavelength range of 536–544nm and the change in blue shift helps in determining the protein concentration.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)