18248

Protein-Disulfid-Isomerase aus Rinderleber

≥150 U/mg protein (protein ca. 40%)

• Synonym(e): PDI
• CAS-Nummer: 37318-49-3
• UNSPSC Code: 12352204
• EG-Nummer: 5.3.4.1 (BRENDA, IUBMB)
• MDL number: MFCD00163364

Eigenschaften

• form: powder, powder with small lumps
• specific activity: ≥150 U/mg protein (protein ca. 40%)
• mol wt: 107 kDa by gel filtration, 57 kDa by SDS-PAGE
• shipped in: wet ice
• storage temp.: −20°C
• InChI: 1S/C7H5Cl2NO5S/c8-4-2-5(9)6(16(13,14)15-10)1-3(4)7(11)12/h1-2H,10H2,(H,11,12)
• InChI key: DHUYKLYJBKXDBM-UHFFFAOYSA-N

Beschreibung

General description

Protein Disulfide Isomerase (PDI) from bovine liver is a homodimer with a molecular weight of 107 kDa (gel filtration) and the molecular weight of the monomer has been reported at 57 kDA (SDS-PAGE). The enzyme is a glycoprotein with 12% total carbohydrate content, composed of 4.6% mannose, 2.5% galactose, 1.4% NANA, and 3.5% 2-acetamido-2-deoxyglucose.

Other Notes

1 U entspricht der Enzymmenge, die in einem Glutathion/Dithiothreit-System eine Absorptionsänderung von 0.001 pro Minute bei pH 4.0 und 25°C bewirkt.

Enzyme that accelerates the exchange reaction between disulfide bridges in proteins