00185
Acetamid-Nährbouillon
NutriSelect® Basic, powder, selective by defined nutrients for Pseudomonas aeruginosa, pack of 500 g, suitable for microbiology
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Manufacturer/tradename:
NutriSelect® Basic
Form:
powder
Suitability:
selective by defined nutrients for Pseudomonas aeruginosa
Application(s):
bioburden testing
environmental
food and beverages
water monitoring, microbiology
environmental
food and beverages
water monitoring, microbiology
Technischer Dienst
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Unterstützung erhaltensterility
non-sterile
Quality Segment
product line
BioChemika
form
powder
shelf life
limited shelf life, expiry date on the label
packaging
pack of 500 g
manufacturer/tradename
NutriSelect® Basic
technique(s)
microbe id | utilization test: suitable
final pH
7.0±0.2 (25 °C)
application(s)
bioburden testing
environmental
food and beverages
water monitoring, microbiology
suitability
selective by defined nutrients for Pseudomonas aeruginosa
Application
Synthetisches Medium zur Detektion des mikrobiologischen Verbrauchs von Acetamid. Entspricht den Empfehlungen des "Deutschen Institut für Normung (DIN)" und den Testprozeduren aus §35 LMBG für die Untersuchung von Wasser auf Kontamination durch Pseudomonas aeruginosa.
Preparation Note
Directions:
Dissolve 0.56 g Acetamide Nutrient Broth Part A and 2 g Acetamide in 950 ml distilled water. Check the pH and if necessary, adjust. Make up to 1 litre and pour into tubes. Autoclave at 121°C for 15 minutes.
Technique:
Medium is inoculated with a couple off loops from the culture to be assayed and it is incubated at 32-35°C for 24-48 hours before going to the isolation medium.
To confirm Ps. aeruginosa, inoculate a loop of culture in Asparagine Proline Broth (Cat. No. 17129) and incubate at 35-37°C for 24 hours. After this period, pour 1-2 drops of Nessler′s Reagent
(Cat. No. 72190) on to the culture and verify ammonia production: a change to yellow will mean ammonia production positive and therefore, Ps. aeruginosa presence.
Dissolve 0.56 g Acetamide Nutrient Broth Part A and 2 g Acetamide in 950 ml distilled water. Check the pH and if necessary, adjust. Make up to 1 litre and pour into tubes. Autoclave at 121°C for 15 minutes.
Technique:
Medium is inoculated with a couple off loops from the culture to be assayed and it is incubated at 32-35°C for 24-48 hours before going to the isolation medium.
To confirm Ps. aeruginosa, inoculate a loop of culture in Asparagine Proline Broth (Cat. No. 17129) and incubate at 35-37°C for 24 hours. After this period, pour 1-2 drops of Nessler′s Reagent
(Cat. No. 72190) on to the culture and verify ammonia production: a change to yellow will mean ammonia production positive and therefore, Ps. aeruginosa presence.
Other Notes
Composition
Part A (Cat. No. 62192-109.4-KC)
Magnesium sulfate 0.158g/L
Sodium chloride 0.2g/L
Sodium molybdate 0.005g/L
Ferrous sulfate 0.0005g/L
Dipotassium hydrogen phosphate 0.2g/L
Part B (Cat. No. 00160-390.6G-KC)
Acetamide 2.0g/L
Part A (Cat. No. 62192-109.4-KC)
Magnesium sulfate 0.158g/L
Sodium chloride 0.2g/L
Sodium molybdate 0.005g/L
Ferrous sulfate 0.0005g/L
Dipotassium hydrogen phosphate 0.2g/L
Part B (Cat. No. 00160-390.6G-KC)
Acetamide 2.0g/L
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Decomposition of acetamide as taxonomic marker for some species of the genus Pseudomonas; Comparative studies on the enrichment of P. aeruginosa
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Warning
hcodes
Hazard Classifications
Carc. 2
Lagerklasse
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
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