ST1200

Anti-Ubiquitin Mouse mAb (FK2)

liquid, clone FK2, Calbiochem^®

• Synonym(e): Mouse Anti-Ubiquitin, Ubiquitin Detection Antibody
• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: FK2, monoclonal
• Species reactivity: -
• Application: —
• Citations: 53

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• antibody form: unpurified
• antibody product type: primary antibodies
• clone: FK2, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity (predicted by homology): all
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• dilution: (ELISA ; Immunoblotting (1:1000); Immunoprecipitation (s and comments); Immunofluorescence )
• isotype: IgG1
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Beschreibung

General description

Mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes K²⁹-, K⁴⁸-, and K⁶³-linked polyubiquitinylated and monoubiquitinylated proteins.

Recognizes K²⁹-, K⁴⁸-, and K⁶³-linked polyubiquitinylated and monoubiquitinylated proteins, but not free ubiquitin, in Raji cells.

This Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin.

Immunogen

human

unpurified polyubiquitinylated lysozyme

Application

ELISA (see application references)
Immunoblotting (1:1000)
Immunoprecipitation (see application references and comments)
Immunofluorescence (see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C). Store diluted antibody at 4°C for up to 1 month.

Raji cells

Other Notes

Does not recognize free ubiquitin. For immunoprecipitation, once the antibody is immobilized, it demonstrates affinity for free ubiquitin as well as for poly- and monoubiquitinylated proteins. Antibody should be titrated for optimal results in individual systems.

Matsumoto, M., et al. 2005. Proteomics 5, 4545.
Boname, J.N., et al. 2001. Immunity 15, 627.
Campbell, V., et al. 2001. Neuron 32, 1013.
Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable