ST1200
Anti-Ubiquitin Mouse mAb (FK2)
liquid, clone FK2, Calbiochem®
Synonym(e):
Mouse Anti-Ubiquitin, Ubiquitin Detection Antibody
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About This Item
UNSPSC-Code:
12352203
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
mouse
Qualitätsniveau
Antikörperform
unpurified
Antikörper-Produkttyp
primary antibodies
Klon
FK2, monoclonal
Form
liquid
Enthält
≤0.1% sodium azide as preservative
Speziesreaktivität (Voraussage durch Homologie)
all
Hersteller/Markenname
Calbiochem®
Lagerbedingungen
OK to freeze
avoid repeated freeze/thaw cycles
Isotyp
IgG1
Versandbedingung
wet ice
Lagertemp.
−20°C
Posttranslationale Modifikation Target
unmodified
Allgemeine Beschreibung
Mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes K29-, K48-, and K63-linked polyubiquitinylated and monoubiquitinylated proteins.
Recognizes K29-, K48-, and K63-linked polyubiquitinylated and monoubiquitinylated proteins, but not free ubiquitin, in Raji cells.
This Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin.
Immunogen
human
unpurified polyubiquitinylated lysozyme
Anwendung
ELISA (see application references)
Immunoblotting (1:1000)
Immunoprecipitation (see application references and comments)
Immunofluorescence (see application references)
Verpackung
Please refer to vial label for lot-specific concentration.
Warnhinweis
Toxicity: Standard Handling (A)
Physikalische Form
In PBS.
Angaben zur Herstellung
Raji cells
Rekonstituierung
Following initial thaw, aliquot and freeze (-20°C). Store diluted antibody at 4°C for up to 1 month.
Sonstige Hinweise
Does not recognize free ubiquitin. For immunoprecipitation, once the antibody is immobilized, it demonstrates affinity for free ubiquitin as well as for poly- and monoubiquitinylated proteins. Antibody should be titrated for optimal results in individual systems.
Matsumoto, M., et al. 2005. Proteomics 5, 4545.
Boname, J.N., et al. 2001. Immunity 15, 627.
Campbell, V., et al. 2001. Neuron 32, 1013.
Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.
Boname, J.N., et al. 2001. Immunity 15, 627.
Campbell, V., et al. 2001. Neuron 32, 1013.
Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.
Rechtliche Hinweise
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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