MABN720

Anti-TBX1 Antibody, clone 7E12.1

culture supernatant, clone 7E12.1, from mouse

• Synonym(e): T-box transcription factor TBX1, T-box protein 1, Testis-specific T-box protein
• UNSPSC Code: 12352203
• NACRES: NA.43
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 7E12.1, monoclonal
• Application: WB
• Citations: -

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: 7E12.1, monoclonal
• species reactivity: human, mouse
• technique(s): western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_542377
• UniProt accession no.: O43435
• target post-translational modification: unmodified
• Gene Information: human ... TBX1(6899)

Beschreibung

General description

T-box transcription factor TBX1 (UniProt O43435; also known as T-box protein 1, Testis-specific T-box protein) is encoded by the TBX1 (also known as CTHM, DGS, TBX1C, VCFS) gene (Gene ID 6899) in human. TBX1 blongs to the T-box family of transcription factors that play important roles in the formation of tissues and organs during embryonic development. TBX1 is required for the second heart field (SHF) development for outflow tract development by regulating proliferation and differentiation of SHF cells. TBX1 is also shown to negatively regulate thymic epithelial cell (TEC) growth and differentiation in mice, extinction of Tbx1 gene expression in the third pharyngeal pouch (3rd pp) endoderm is a prerequisite for thymus organogenesis. Human TBX mutations cause DiGeorge syndrome, characterized by parathyroid hypoplasia, thymic hypoplasia, defects in the outflow tract of the heart, and craniofacial anomalies.

~48 kDa observed. 43.13 kDa (isoform A), 40.35 kDa (isoform B), and 52.67 kDa (isoform C; TBX1C) calculated. Uncharacterized band(s) may appear in some lysates.

Immunogen

Epitope: C-terminal region.

GST-tagged recombinant human TBX1 C-terminal fragment.

Application

Anti-TBX1 Antibody, clone 7E12.1 is an antibody against TBX1 for use in Western Blotting.

Research Category
Neuroscience

Research Sub Category
Transcription Factors

Western Blotting Analysis: A 1:500 dilution from a representative lot detected TBX1 in 10 µg of mouse Neuro-2a and human SH-SY5Y neuroblastoma cell lysates.

Biochem/physiol Actions

Clone 7E12.1 was raised against a recombinant fragment derived from TBX1 spliced isoform A (Uniprot O43435-1), cross-reactivity toward spliced isoform B and isoform C (TBX1C) is possible, but has not been determined.

Physical form

Mouse monoclonal IgG2aκ hybridoma culture supernatant without preservatives.

Unpurified

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Evaluated by Western Blotting in HUVEC lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected TBX1 in 10 µg of HUVEC lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Replaces: AB15624

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 2