MABF3201

Anti-MART-1/Melan-A Antibody, clone A103

• Synonym(e): Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, Protein Melan-A
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: A103, monoclonal
• Application: IHC (p), WB
• Citations: -

Eigenschaften

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: A103, monoclonal
• mol wt: calculated mol wt 13.16 kDa, observed mol wt ~16 kDa
• purified by: using protein G
• species reactivity: human
• packaging: antibody small pack of 100 μL
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
• isotype: IgG1κ
• epitope sequence: Unknown
• Protein ID accession no.: NP_005502
• UniProt accession no.: Q16655
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... MLANA(2315)

Beschreibung

General description

Melanoma antigen recognized by T-cells 1 (UniProt: Q16655; also known as MART-1, Antigen LB39-AA, Antigen SK29-AA, Protein Melan-A) is encoded by the MLANA (also known as MART1) gene (Gene ID: 2315) in human. MART-1, a melanosome-specific protein, is a single-pass type III membrane protein with a transmembrane domain (aa 27-47) and a cytoplasmic domain (aa 48-118). Its expression is restricted to melanocytes, melanoma, and retina and is absent in other tissues and tumors. It is highly enriched in early melanosomes (Stage I and/or II melanosomes) indicating that its role in early melanogenesis. It is reported to stabilize both GPCR143/OA-1 and plays a role in the expression, stability, trafficking, and processing of pre-melanosome protein (PMEL) to ensure the formation of a stage 2 melanosome. It is mainly found in the endoplasmic reticulum (ER) and Golgi but is also detected in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation, which allows melanoma cells to be effectively recognized by specific cytotoxic T-cells. MART-1 may be a useful marker in the diagnosis of melanocytic lesions and in the differential diagnosis of malignant melanoma. (Ref.: Hoashi, T., et al. (2005). J. Biol. Chem. 280(14); 14006-14016; Chen, YT., et al. (1996). Proc. Natl. Acad. Sci. USA. 93(12); 5915-5919).

Immunogen

Full-length, recombinant, human MART-1 produced in E.coli.

Application

Quality Control Applications

Evaluated by Western Blotting in SK-MEL-28 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected MART-1 in SK-MEL-28 cell lysate.

Tested applications

Western Blotting Analysis: A representative lot detected MART-1 in Western Blotting applicaiton. (Chen, Y.T., et al. (1996). Proc Natl Acad Sci USA. 93(12); 5915-9).

Immunohistochemistry Applications: A representative lot detected MART-1/Melan-A in Immunohistochemistry applications (Chen, Y.T., et al. (1996). Proc Natl Acad Sci USA. 93(12);5915-9).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Biochem/physiol Actions

Clone A103 is a mouse monoclonal antibody that detects MART-1.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 13 - Non Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable