MABF244

Anti-PAR-1, clone ATAP2 Antibody

clone ATAP2, from mouse

• Synonym(e): Proteinase-activated receptor 1, PAR-1, Coagulation factor II receptor, Thrombin receptor
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: ATAP2, monoclonal
• Application: FACS, ICC, IF, WB
• Citations: 6

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: ATAP2, monoclonal
• species reactivity: human, mouse
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001983
• UniProt accession no.: P25116
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... F2R(2149)

Beschreibung

General description

The protein named Proteinase-activated receptor 1 (PAR-1/PAR1) or Coagulation factor II receptor, or Thrombin receptor and encoded by the human gene F2R/CR2R/PAR1/TR is a high affinity receptor for activated thrombin and part of the innate immune system response driven by proteinase-activated receptors (PARs). PARs are G protein-coupled receptors that transmit cellular responses to extracellular proteases and have important functions in vascular physiology, development, inflammation, and cancer progression. PAR1 is important for platelet activation and vascular development. PAR1 expression is limited to platelets and vascular endothelial cells and is localized to the cell membrane. Interestingly too, even the signal peptide of PAR1 (which guides the protein to the membrane) plays a role in cell signaling as if forms the intracellular angiogenesis inhibitor peptide Parstatin.

~50 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Immunogen

Linear peptide corresponding to human Par-1.

Application

Flow Cytometry Analysis: 4 µg from a representative lot detected Par-1 in 1X10E6 human thrombocytes (platelets).

Flow Cytometry Analysis: A representative lot from an independent laboratory detected Par-1 in CHRF-288 and HEL cells (Hoxie, J. A., et al. (1993). J Biol Chem. 268(18):13756-13763.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Par-1 in human platelet membrane cell lysate (Brass, L. F., et al. (1992). J Biol Chem. 267(20):13795-13798.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Par-1 in CHRF-288 and HEL cells (Hoxie, J. A., et al. (1993). J Biol Chem. 268(18):13756-13763.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected Par-1 in CHRF-288 cells (Brass, L. F., et al. (1994). J Biol Chem. 269(4):2943-2952.).

This Anti-PAR-1 antibody, clone ATAP2 is validated for use in western blotting, flow cytometry, ICC & immunofluorescence for the detection of PAR-1.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in mouse lymph node tissue.

Western Blotting Analysis: 1 µg/mL of this antibody detected Par-1 in 10 µg of mouse lymph node tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable