MABF2128

Anti-Properdin Antibody, clone 6E11A4

clone 6E11A4, from mouse

• Synonym(e): Complement factor P
• UNSPSC Code: 12352203
• NACRES: NA.43
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 6E11A4, monoclonal
• Application: ELISA, IHC, WB
• Citations: -

Eigenschaften

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 6E11A4, monoclonal
• species reactivity: baboon, rabbit, canine, human
• packaging: antibody small pack of 25 μg
• technique(s): ELISA: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001138724
• UniProt accession no.: P27918
• target post-translational modification: unmodified
• Gene Information: human ... CFP(5199)

Beschreibung

General description

Properdin (UniProt: P27918; also known as Complement factor P) is encoded by the CEP (also known as PFC) gene (Gene ID: 5199) in human. (ABF185: Properdin (UniProt P27918; also known as Complement factor P) is encoded by the CFP (also known as PFC, PFD) gene (Gene ID 5199) in human. Properdin is a plasma glycoprotein and the only known positive regulator of the complement cascade. It binds and stabilizes the alternative pathway (AP) C3 convertase C3bBb and may also serve as a platform to form new C3bBb convertases on cell surface. Properdin-mediated AP activation plays an important role in host defense, and properdin deficiency (PFD) increases the risk for Neisseria meningitidis infection in human. Properdin-deficiency exacerbated disease condition in a murine model of factor H- (fH-) related C3 glomerulopathy, but prevented the development of allergen-induced airway inflammation. Human properdin is produced with a signal peptide sequence (a.a. 1-27), which is removed posttranslationally to yield the mature protein (a.a. 28-469) composed entirely of seven TSP type-1 domains (domain 0 through 6; a.a. 28-462). TSP type-1 domains 0 and 6 bind to each other and mediate multimerization.

~51 kDa observed; 51.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Application

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Properdin in human tonsil tissue sections.

ELISA Analysis: A representative lot detected Properdin in ELISA applications (Blatt, A.Z., et. al. (2016). J Immunol. 196(11):4671-4680).

Inhibition Analysis: A representative lot detected Properdin in Inhibition applications (Blatt, A.Z., et. al. (2016). J Immunol. 196(11):4671-4680).

This mouse monoclonal Anti-Properdin, clone 6E11A4 Antibody, Cat. No. MABF2128 is tested for use in Western Blotting, ELISA, and Immunohistochemistry (Paraffin), for the detection of Properdin. Inhibits function in Properdin.

Biochem/physiol Actions

Clone 6E11A4 specifically detects human properdin.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in purified human properdin.

Western Blotting Analysis: 1 µg/mL of this antibody detected Properdin in purified human properdin.

Other Notes

Concentration: Please refer to lot specific datasheet.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable