MABE944

Anti-phospho Histone H3 (Thr32), clone 6C7G12 Antibody

clone 6C7G12, from rat

• Synonym(e): Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/I, Histone H3/j, Histone H3/k, Histone H3/l
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rat
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 6C7G12, monoclonal
• Speziesreaktivität: human
• Methode(n): ChIP: suitable (ChIP-seq); ELISA: suitable; immunocytochemistry: suitable; western blot: suitable
• Isotyp: IgG2aκ
• NCBI-Hinterlegungsnummer: NP_002098
• UniProt-Hinterlegungsnummer: P84243
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: phosphorylation (pThr32)
• Angaben zum Gen: human ... HIST1H3F(8968)

Beschreibung

Allgemeine Beschreibung

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Acetylation of lysines in histone H3 is associated with genes that are being actively transcribed into RNA.

Immunogen

KLH-conjugated linear peptide corresponding to phospho Histone H3 (Thr32).

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
Histone

Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody was reported to be suitable for ChIP. Personal communication Dr. T. Tachibana, Cell Engineering Corp. Osaka City, Japan.

ChIP-seq: representative lot of this antibody was reported to be suitable for ChIP-seq. Personal communication Dr. T. Tachibana, Cell Engineering Corp. Osaka City, Japan.

Immunocytochemistry: A representative lot of this antibody was reported to detect histone H3 phosphorylated at threonine 32 in Mitotic DM4 cells. See Yoshimi et al. (2013).

ELISA: A representative lot of this antibody was used in ELISA against a panel of phophorylated and unmodified peptides to demonstrate specificity. See Yoshimi et al. (2013).

This Anti-phospho Histone H3 (Thr32), clone 6C7G12 Antibody is validated for use in Western Blotting and ELISA and Immunocytochemistry and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of phospho Histone H3 (Thr32).

Qualität

Evaluated by Western Blotting in a nocodozole treated HeLa cell lysate.

Western Blotting Analysis: A 2 µg/mL dilution of this antibody detected phospho histone H3 (Thr32) in a nocodozole treated HeLa cell lysate.

Zielbeschreibung

~17 kDa observed

Physikalische Form

Protein G Purified

Format: Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable