MABE1784

Anti-NCoA-2 Antibody, clone 3Ti-3C11

• Synonym(e): Class E basic helix-loop-helix protein 75 (bHLHe75), NCoA-2, SRC2, Transcriptional intermediary factor 2 (hTIF2)
• NACRES: NA.43
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: 3Ti-3C11, monoclonal
• Application: ChIP, ELISA, WB
• Citations: -

Eigenschaften

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 3Ti-3C11, monoclonal
• mol wt: calculated mol wt 159 kDa, observed mol wt ~85 kDa
• purified by: using protein G
• species reactivity: mouse, human
• species reactivity (predicted by homology): feline, horse
• packaging: antibody small pack of 100
• technique(s): ChIP: suitable, ELISA: suitable, western blot: suitable
• isotype: IgG1κ
• epitope sequence: N-terminal half
• Protein ID accession no.: NP_001308632
• UniProt accession no.: Q15596
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... NCOA2(10499)

Beschreibung

General description

Nuclear receptor coactivator 2 (UniProt: Q15596; also known as NCoA-2, Transcriptional intermediary factor 2, hTIF2, Class E basic helix-loop-helix protein 75 (bHLHe75)) is encoded by the NCOA2 (also known as BHLHE75, SRC2, TIF2) gene (Gene ID: 10499) in human. Nuclear receptor coactivator 2 (NCoA-2) functions as a transcriptional coactivator in various cellular processes. It belongs to the p160/steroid receptor coactivator (SRC) family of proteins. NCoA-2 plays a crucial role in the regulation of gene expression by interacting with nuclear receptors and other transcription factors. It possesses multiple functional domains, including a nuclear receptor interaction domain (NRID), a histone acetyltransferase (HAT) domain, and several transcription activation domains (ADs). NCoA-2 acts as a bridge between transcription factors and the transcriptional machinery, facilitating the recruitment of coactivators and chromatin remodeling complexes to target gene promoters. This coactivator function enables NCoA-2 to modulate diverse biological processes such as development, metabolism, cell proliferation, and differentiation. Dysregulation of NCoA-2 has been implicated in various diseases, including cancer, where it can promote tumor growth and progression.

Immunogen

His-tagged, recombinant, human NCoA-2 protein fragment (aa 624-1287).

Application

Quality Control Testing

Evaluated by Western Blotting with His-tagged, recombinant fragment of Human NCoA-2.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected His-tagged, recombinant fragment (aa 624-1287) of Human NCoA-2.

Tested Applications

ELISA Analysis: A representative lot detected His-tagged, recombinant fragment (aa 624-1287) of Human NCoA-2.

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected NCoA-2 in Chromatin Immunoprecipitation application. (Rambaud, J., et al. (2009). J Biol Chem. 284(21):14147-56; Duteil, D., et al. (2010). Cell Metab. 12(5):496-508; Ganti, K.P., et al. (2017). Proc Natl Acad Sci USA. 114(6):E951-E960).

Western Blotting Analysis: A representative lot detected NCoA-2 in Western Blotting application. (Rambaud, J., et al. (2009). J Biol Chem. 284(21):14147-56; Duteil, D., et al. (2010). Cell Metab. 12(5):496-508).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Biochem/physiol Actions

Clone 3Ti-3C11 mouse monoclonal antibody that detects NCoA-2. It targets an epitope within 246 amino acids from the internal part of the protein.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable