MABE150

Anti-KDM5B Antibody, clone 15G8.1

clone 15G8.1, from mouse

• Synonym(e): Lysine-specific demethylase 5B, KDM5B, Cancer/testis antigen 31, CT31, Histone demethylase JARID1B, Jumonji/ARID domain-containing protein 1B, PLU-1, Retinoblastoma-binding protein 2 homolog 1, RBP2-H1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 15G8.1, monoclonal
• Application: IHC, WB
• Citations: -

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 15G8.1, monoclonal
• species reactivity: human, mouse
• technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_006609
• UniProt accession no.: Q9UGL1
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... KDM5B(10765)

Beschreibung

General description

Lysine-specific demethylase 5B (UniProt Q9UGL1; also known as Cancer/testis antigen 31, CT31, Histone demethylase JARID1B, Jumonji AT rich interactive domain 1B, Jumonji/ARID domain-containing protein 1B, PLU-1, Protein phosphatase 1 regulatory subunit 98, Putative DNA/chromatin binding motif, RBP2-H1, Retinoblastoma-binding protein 2 homolog 1) is encoded by the KDM5B (also known as PLU1, PUT1, JARID1B, PPP1R98, RBBP2H1, RBBP2H1A) gene (Gene ID 10765) in human. JARID1B/KDM5B is an H3K4me2/3 demethylase that regulates self-renewal and differentiation in a cell type-specific manner. JARID1B/KDM5B is highly expressed in embryonic stem cells (ESCs), hematopoietic stem cells (HSCs), and in various primary cancers including melanoma, breast, testicular, and is upregulated in leukemic cell lines.

~175 kDa observed. Uncharacterized band(s) may appear in some lysates.

Immunogen

Epitope: Region between ARID and JmjC domain

GST-tagged recombinant protein corresponding to the region between ARID and JmjC domain of human KDM5B.

Application

This Anti-KDM5B Antibody, clone 15G8.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of KDM5B.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected KDM5B in 10 µg of MCF-7 cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected KDM5B in human kidney and mouse cerebral cortex tissue.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected KDM5B in 10 µg of HeLa cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable