MAB8129

Anti-Cytomegalovirus Antibody, immediate early, clone 7D12.2

clone 7D12.2, Chemicon^®, from mouse

• Synonym(e): CMV
• UNSPSC Code: 12352203
• NACRES: NA.42
• eCl@ss: 32160702
• Clone: 7D12.2, monoclonal
• Species reactivity: human
• Application: ICC, IF, IHC, WB
• Citations: 1

Eigenschaften

• biological source: mouse
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 7D12.2, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG1
• suitability: not suitable for immunohistochemistry (Paraffin)
• shipped in: wet ice

Beschreibung

Immunogen

Epitope: immediate early

MRC-5 cells infected with CMV AD169.

Application

Immunohistochemistry.

Immunocytochemistry.

IFA at 1:3,000-1:6,000 on acetone fixed cells.

Does not work on paraffin embedded tissue sections.

Dilute with buffer pH 7.4-7.6 to desired working volume.

For extensive dilution, protein containing or other stabilizing medium should be used.

Final working dilutions must be determined by end user.

Research Category
Infectious Diseases

Research Sub Category
Infectious Diseases - Viral

This Anti-Cytomegalovirus Antibody, immediate early, clone 7D12.2 is validated for use in IF, WB, IC, IH for the detection of Cytomegalovirus.

Biochem/physiol Actions

Reacts with an immediate early protein M.W. 68 kD. Can detect CMV infection 1 hour post-infection exhibiting a nuclear and cytoplasmic staining which reaches peak intensity between 8-24 hours.

With CMV the antigens expressed at different times are listed as:



Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.

Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.

Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD

Physical form

Format: Purified

Purified ascites in 0.02 M Phosphate Buffered Saline, 0.25 M Sodium Chloride, 0.1% Sodium Azide,

pH 7.6 .

Preparation Note

Maintain at +2° to + 8°C for up to 12 months from date of receipt.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 2