MAB5210

Anti-Phosphacan Antibody, clone 122.2

ascites fluid, clone 122.2, Chemicon^®

• Synonym(e): Receptor Tyrosine Phosphatase beta
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 122.2, monoclonal
• Application: ICC, IHC, WB
• Citations: 7

Eigenschaften

• biological source: mouse
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 122.2, monoclonal
• species reactivity: rat
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgM
• UniProt accession no.: Q62656
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... PTPRZ1(5803)

Beschreibung

General description

Phosphacan is a soluble nervous tissue-specific proteoglycan that is thought to regulate axonal outgrowth. It is synthesized by glia and binds to neurons and to the neural cell adhesion molecules tenascin, N-CAM or NG-CAM but not to laminin and fibronectin. Phosphacan acts as a potent inhibitor of cell adhesion and neurite outgrowth.

Application

Anti-Phosphacan Antibody, clone 122.2 detects level of Phosphacan & has been published & validated for use in WB, IC, IH.

Research Category
Neuroscience

Research Sub Category
Growth Cones & Axon Guidance

Western blot. Recognizes bands at 250, 190, and 180 kDa on western blots of embryonic rat brain extracts.

Immunocytochemistry: 1:10

Immunohistochemistry on 4% paraformaldehyde fixed tissue: 1:1,000

Immunoprecipitation

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Phosphacan (Receptor Tyrosine Phosphatase Beta). Recognizes the core protein.

Physical form

Ascites fluid. Liquid. Contains no preservative.

Preparation Note

Maintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
POSITIVE CONTROL:

embryonic or early post-natal rat brain.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable