MAB3738

Anti-PML Antibody, clone 36.1-104

ascites fluid, clone 36.1-104, Chemicon^®

• Synonym(e): Promyelocytic Leukemia Protein, Tripartite Motif Protein 19
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 36.1-104, monoclonal
• Application: ICC, IP, WB
• Citations: 34

Eigenschaften

• biological source: mouse
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 36.1-104, monoclonal
• species reactivity: mouse
• should not react with: human
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG2b
• NCBI accession no.: NM_002675.3, NM_033238.2, NM_033239.2, NM_033240.2, NM_033244.3, NM_033246.2, NM_033247.2, NM_033249.2, NM_033250.2
• UniProt accession no.: P29590
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... PML(5371)

Beschreibung

General description

106 kDa

PML protein is a tripartite motif (TRIM)-containing nuclear protein that may function as, among other things, a transcription factor, a coactivator of nuclear receptors (Ruggerio, 2000), a regulator of apoptosis (Guo, 2000), a mediator of interferon-induced antiviral response (Regand and Chelbi-Aliz, 2001), and as a suppressor of growth and oncogenic transformation (Mu, 1997). PML is localized to the nucleoplasm and in distinct subnuclear structures referred to as Promyelocytic Leukemia Bodies (also known as Nuclear Domain 10). Localization of PML to Promyelocytic Leukemia Bodies requires modification of PML protein by the Small Ubiquitin Modifier (SUMO) and is required for proper formation and integrity of these subnuclear structures. At least 14 splice variants of PML ranging in molecular weight from 48-97 kDa (predicted) have been described in the literature. The functional significance of the various splice variants is not well understood. In patients with Acute Promyelocytic Leukemia, the PML gene is involved in at least two specific chromosomal translocations that result in the expression of chimeric proteins with the Retinoic Acid Receptor alpha (RARalpha DNA- and Hormone-binding domains; Pandolfi, 2001). All isoforms of PML, as well as the PML-RARalpha chimeric proteins expressed in Type A and Type B APL contain an identical N-terminus but vary in the C-terminal portion of the protein (Jenson, 2001).

Immunogen

His-tagged PML fusion protein corresponding to amino acids 1-581 of mouse PML.

Application

Anti-PML Antibody, clone 36.1-104 is a Mouse Monoclonal Antibody for detection of PML also known as Promyelocytic Leukemia Protein & has been validated in ICC, IP & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Western blot (1:500)

Immunoprecipitation

Immunocytochemistry (1:100)

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Recognizes mouse PML (Promyelocytic Leukemia protein). On western blots of protein extracts from mouse embryo fibroblast (MEF1 cells), MAB3738 recognizes a band migrating at approximately 106 kDa corresponding to PML.

Physical form

Ascites fluid containing no preservatives.

Unpurified

Preparation Note

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
POSITIVE CONTROL: Mouse embryo fibroblasts (MEF1 cells).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable