MAB2148-C

Anti-PECAM-1 Antibody, clone P2B1 (Ascites Free)

clone P2B1, 1 mg/mL, from mouse

• Synonym(e): Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA′, PECA1, CD31
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: P2B1, monoclonal
• Application: ELISA, FACS, ICC, IF, IHC, IP
• Citations: 2

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: P2B1, monoclonal
• species reactivity: human
• concentration: 1 mg/mL
• technique(s): ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable
• isotype: IgG1κ
• NCBI accession no.: NP_000433
• UniProt accession no.: P16284
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PECAM1(5175)

Beschreibung

General description

PECAM-1 is a type I integral membrane glycoprotein, and a member of the immunoglobulin superfamily of cell surface receptors. PECAM-1 is constitutively expressed on the surface of endothelial cells where it is concentrated at cell junctions. PECAM-1 is also expressed at low levels on all leukocytes and platelets. Homotypic interaction of CD31 is important in cell-cell interaction, cell-matrix interaction and signal transduction.

~80 kDa calculated

Immunogen

Whole cells expressing human PECAM-1.

Application

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected PECAM-1 in pluripotent cells (Boyd, N. L., et al. (2007). Exp Biol Med. 232(6):833-843.).

Immunoprecipitation Analysis: A representative lot detected PECAM-1 by immunoprecipitation.

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected PECAM-1 in astrocytoma tissue sections (Bronger, H., et al. (2005). Cancer Res. 65(24):11419-28.).

ELISA Analysis: A representative lot of from an independent laboratory detected PECAM-1 in a panel of CD31 mutant cell lines (Newton, J. P, et al. (1997) J. Biol. Chem., 272: 20555-63.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected PECAM-1 in astrocytoma tissue sections (Bronger, H., et al. (2005). Cancer Res. 65(24):11419-28.).

Research Category
Cell Structure

Research Sub Category
ECM Proteins

This Anti-PECAM-1 Antibody, clone P2B1 (Ascites Free) is validated for use in flow cytometry, ICC, IP, IHC, ELISA & immunofluorescence for the detection of PECAM-1.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Flow Cytometry in human PBMCs.

Flow Cytometry Analysis: 1 µg of this antibody detected PECAM-1 in 1X10E6 human PBMCs.

Other Notes

Replaces: 04-1074, MAB2148

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1