MAB1957

Anti-Integrin beta 3 (ITGB3/CD61) Antibody

CHEMICON^®, mouse monoclonal, 25E11

• Synonym(e): CD61
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: 25E11, monoclonal
• Species reactivity: human
• Application: FACS, ICC, IP
• Citations: 24

Eigenschaften

• Produktname: Anti-Integrin β3 Antibody, clone 25E11, clone 25E11, Chemicon^®, from mouse
• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 25E11, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable
• isotype: IgM
• NCBI accession no.: NM_000212.2
• UniProt accession no.: P05106
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ITGB3(3690)

Beschreibung

Immunogen

Normal blood mononuclear cells activated by mixed lymphocyte reaction and cultured for 10 days in medium containing IL-2.

Application

Immunoprecipitation

Immunocytochemistry (acetone fixation required)

Flow cytometry

Inhibits platelet aggregation, 10 μg/mL

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

This Anti-Integrin β3 Antibody, clone 25E11 is validated for use in FC, IP, IC, FUNC for the detection of Integrin β3.

Biochem/physiol Actions

Reacts with human integrin beta3 (GPIIIa, vitronectin receptor beta chain). The protein detectable is a complex of CD41 and CD61. The apparent molecular weight of the GPIIIa by SDS-PAGE is 105 kDa reduced and 90 kDa unreduced. Ligands are fibronectin, fibrinogen, von Willebrand factor, vitronectin and thrombospondin. Residues 237-248 of GPIIIa or CD61 are critical in adhesive protein binding. The monocytoid cell line U937 is highly positive.

(original paper claimed IgG2ak)

Physical form

Format: Purified

In buffer of 0.02M phosphate, 0.25M sodium chloride, pH 7.6, 0.1% sodium azide.

Preparation Note

Maintain between 2 and 8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-1060

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• Lagerklasse: 12 - Non Combustible Liquids