MAB1820

Anti-Scribble Antibody

clone 7C6.D10, Chemicon^®, from mouse

• Synonym(e): Scrib, LAP4
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 7C6.D10, monoclonal
• Application: WB
• Citations: 6

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 7C6.D10, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): western blot: suitable
• isotype: IgG1κ
• GenBank accession no.: AAL38976
• UniProt accession no.: Q14160
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... SCRIB(23513)

Beschreibung

General description

220 kDa

Scribble is a tumor suppressor marker first discovered in Drosophila that has been implicated in the control of cell proliferation, polarity, invasion, and metastasis (Humbert, et al, 2003). Mammalian Scribble expresses basolateral staining in epithelial tissue and highly polarized expression in T lymphocytes (Dow, et al, 2003) (Ludford-Menting, et al, 2005), and plays an essential role in the regulation of the polarity specifically involved in directed epithelial migration. Altered expression of human Scribble is associated with invasive epithelial cancers, however, its role in tumour development remains unclear (Dow, et al, 2007).

Immunogen

Epitope: PDZ2 domain of Scribble

GST-tagged fusion protein corresponding to the PDZ2 domain of Scribble

Application

Suggested dilutions:
Western blot: 0.2 µg (1:2000)

Research Sub Category
Tumor Markers

Cytoskeleton

This Anti-Scribble Antibody is validated for use in WB for the detection of Scribble.

Biochem/physiol Actions

Predicted to cross react with mouse based on sequence homology. Reactivity with other species has not been tested.

Physical form

Format: Purified

Purified in PBS containing with 0.05% NaN3.

Preparation Note

Maintain at 2-8°C for up to 1 year after date of receipt.

Analysis Note

Routinely evaluated by Western blot on HeLa cell lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

GenBank is a registered trademark of United States Department of Health and Human Services

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable