MAB13170

Anti-Cav1.2 calcium channel Antibody, clone L57/46

clone L57/46, from mouse

• Synonym(e): calcium channel, voltage-dependent, L type, alpha 1C subunit1, voltage-gated calcium channel alpha subunit Cav1.2, calcium channel, L type, alpha 1 polypeptide, isoform 1, cardic muscle, calcium channel, cardic dihydropyridine-sensitive, alpha-1 subunit,
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: L57/46, monoclonal
• Application: IHC, WB
• Citations: 6

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: L57/46, monoclonal
• species reactivity: mouse, human
• species reactivity (predicted by homology): rabbit (based on 100% sequence homology), rat (based on 100% sequence homology)
• technique(s): immunohistochemistry: suitable, western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_001129994
• UniProt accession no.: P15381
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CACNA1C(775) ; rabbit ... Cacna1C(100101555)

Beschreibung

General description

Observed at ~ 240 kDa

This gene encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. There are multiple isoforms of each of these proteins, either encoded by different genes or the result of alternative splicing of transcripts. The protein encoded by this gene binds to and is inhibited by dihydropyridine. Many alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Immunogen

Epitope: Cytoplasmic domain

Recombinant protein corresponding to rabbit Cav1.2 calcium channel at the cytoplasmic domain proximal to the C-terminus.

Application

Immunohistochemistry Analysis: 1:300 dilution of this antibody has been shown to detect Cav1.2 calium channel in human kidney tissue.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

This Anti-Cav1.2 calcium channel Antibody, clone L57/46 is validated for use in WB, IH for the detection of Cav1.2 calcium channel.

Biochem/physiol Actions

This antibody recognizes Cav1.2 calcium channel at the cytoplasmic domain.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain tissue lysate

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected Cav1.2 calcium channel on 10 µg of mouse brain tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable