MAB1273B

Anti-Mitochondria Antibody, clone 113-1, Biotin Conjugate

clone 113-1, from mouse, biotin conjugate

• Synonym(e): Human Mitochondria
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: biotin conjugate
• Clone: 113-1, monoclonal
• Application: ICC, IHC
• Citations: 2

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• conjugate: biotin conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 113-1, monoclonal
• species reactivity: human (mitochondria protein)
• should not react with: rat (mitochondria protein), mouse (mitochondria protein)
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable
• isotype: IgG1
• shipped in: wet ice
• target post-translational modification: unmodified

Beschreibung

General description

60 kDa calculated

This antibody recognizes a 60 kDa nonglycosylated protein component of mitochondria found in human cells. It can be used to stain mitochondria in cell, tissue or biochemical preparations and can be used as a marker of the mitochondria in subcellular fractions. MAB1273B recognizes a 65 kD protein by immunoprecipitation and produces the stringy, "spaghetti-like", staining patten in the cytoplasm of human cells.

Immunogen

Surface of intact human mitochondria

Application

Detect Mitochondrial Marker using this mouse monoclonal antibody, Anti-Mitochondria Antibody, clone 113-1, Biotin Conjugate validated for use in ICC & IHC.

Evaluated by Immunocytochemistry in primary mouse embryonic fibroblasts (PMEFs).
Immunocytochemsitry Analysis: A 1:50 dilution of this antibody did not detect mitrochondria in primary mouse embryonic fibroblasts (PMEFs). Evaluated by Immunohistochemistry in human kidney tissue.
Immunocytochemsitry Analysis: A 1:500 dilution of this antibody detected mitrochondria in human kidney tissue.

Research Category
Stem Cell Research

Cell Structure

Research Sub Category
Developmental Neuroscience

Organelle & Cell Markers

Biochem/physiol Actions

This antibody recognizes human. Antibody is not reactive with mouse or rodent.

Physical form

Protein G Purified

Purified mouse monoclonal IgG1 conjugated to Biotin in PBS with 0.1% sodium azide and 15mg/ml BSA.

Preparation Note

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
Human adipose mesenchymal stem cells (SCC038).

Evaluated by Immunocytochemistry in human adipose mesenchymal stem cells (SCC038).
Immunocytochemsitry Analysis: A 1:50 dilution of this antibody detected mitrochondria in human adipose mesenchymal stem cells (SCC038).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: WGK 2