For cellular staining using SSEA-1 in flow cytometry, prepare an antibody working solution by diluting the primary antibody 1:5 with PBS. Dispense the volume per test of the working solution according to the number of cells indicated below:
- Use 5 µL for Guava Flow Cytometer with 5x10^5 cells in 95 µL PBS, totaling 100 µL.
- Use 10 µL for other flow cytometry systems with 1x10^6 cells in 90 µL PBS, totaling 100 µL.
Über diesen Artikel
biological source
mouse
Quality Segment
conjugate
PE
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
MC-480, monoclonal
species reactivity
rat, mouse, human
technique(s)
flow cytometry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable
isotype
IgM
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... FUT4(2526)
General description
Immunogen
Application
Stem Cell Research
Pluripotent & Early Differentiation
Biochem/physiol Actions
Physical form
Preparation Note
Analysis Note
Mouse embryonic Stem Cells
Other Notes
Disclaimer
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| description clone MC-480, from mouse, PE | description clone MC-480, Chemicon®, from mouse | description clone MC-480, Chemicon®, from mouse | description clone MC-813-70, Milli-Mark®, from mouse |
| species reactivity rat, mouse, human | species reactivity mouse, human, rat | species reactivity mouse, human, rat | species reactivity human |
| Gene Information human ... FUT4(2526) | Gene Information human ... FUT4(2526) | Gene Information human ... FUT4(2526) | Gene Information human ... FUT4(2526) |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| clone MC-480, monoclonal | clone MC-480, monoclonal | clone MC-480, monoclonal | clone MC-813-70, monoclonal |
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form saturated ammonium sulfate (SAS) precipitated | antibody form purified antibody |
| conjugate PE | conjugate ALEXA FLUOR™ 488 | conjugate unconjugated | conjugate PE |
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Suggest suitable dilaution of SSEA-1 for flow cutometry.
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