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FCMAB117P

Anti-SSEA-1 Antibody, clone MC-480, PE conjugate

clone MC-480, from mouse, PE

Synonym(e):

SSEA-1

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Über diesen Artikel

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
PE
Clone:
MC-480, monoclonal
Application:
FACS, IF, IHC, IP
Citations:
4


biological source

mouse

Quality Segment

conjugate

PE

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MC-480, monoclonal

species reactivity

rat, mouse, human

technique(s)

flow cytometry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

isotype

IgM

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... FUT4(2526)

General description

220 kDa Calculated
SSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.

Immunogen

Epitope: MC-480
F9 tetracarcinoma stem cells (X-irradiated).

Application

Anti-SSEA-1 Antibody, clone MC-480, PE conjugate is an antibody against SSEA-1 for use in IH, IP, IF, FC.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

Biochem/physiol Actions

This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).

Physical form

Purified mouse monoclonal IgM conjugated to PE fluorochrome in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Preparation Note

Maintain refrigerated at 2-8oC in undiluted aliquots for up to 6 months from date of receipt. Protect from light.

Analysis Note

Control
Mouse embryonic Stem Cells
Evaluated by Flow Cytometry with mouse embryonic stem cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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description

clone MC-480, Chemicon®, from mouse

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species reactivity

rat, mouse, human

species reactivity

mouse, human, rat

species reactivity

mouse, human, rat

species reactivity

human

Gene Information

human ... FUT4(2526)

Gene Information

human ... FUT4(2526)

Gene Information

human ... FUT4(2526)

Gene Information

human ... FUT4(2526)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

MC-480, monoclonal

clone

MC-480, monoclonal

clone

MC-480, monoclonal

clone

MC-813-70, monoclonal

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

saturated ammonium sulfate (SAS) precipitated

antibody form

purified antibody

conjugate

PE

conjugate

ALEXA FLUOR™ 488

conjugate

unconjugated

conjugate

PE


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Analysenzertifikate (COA)

Lot/Batch Number

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Questions

  1. Suggest suitable dilaution of SSEA-1 for flow cutometry.

    1 answer
    1. For cellular staining using SSEA-1 in flow cytometry, prepare an antibody working solution by diluting the primary antibody 1:5 with PBS. Dispense the volume per test of the working solution according to the number of cells indicated below:
      - Use 5 µL for Guava Flow Cytometer with 5x10^5 cells in 95 µL PBS, totaling 100 µL.
      - Use 10 µL for other flow cytometry systems with 1x10^6 cells in 90 µL PBS, totaling 100 µL.

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