For cellular staining using SSEA-1 in flow cytometry, prepare an antibody working solution by diluting the primary antibody 1:5 with PBS. Dispense the volume per test of the working solution according to the number of cells indicated below:
- Use 5 µL for Guava Flow Cytometer with 5x10^5 cells in 95 µL PBS, totaling 100 µL.
- Use 10 µL for other flow cytometry systems with 1x10^6 cells in 90 µL PBS, totaling 100 µL.
FCMAB117P
Anti-SSEA-1 Antibody, clone MC-480, PE conjugate
clone MC-480, from mouse, PE
Synonym(e):
SSEA-1
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100 TESTS
€ 370,00
About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
mouse
Qualitätsniveau
Konjugat
PE
Antikörperform
purified immunoglobulin
Antikörper-Produkttyp
primary antibodies
Klon
MC-480, monoclonal
Speziesreaktivität
rat, mouse, human
Methode(n)
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
Isotyp
IgM
NCBI-Hinterlegungsnummer
UniProt-Hinterlegungsnummer
Versandbedingung
wet ice
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
human ... FUT4(2526)
Verwandte Kategorien
Allgemeine Beschreibung
SSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.
Spezifität
This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).
Immunogen
Epitope: MC-480
F9 tetracarcinoma stem cells (X-irradiated).
Anwendung
Research Category
Stammzellforschung
Stammzellforschung
Research Sub Category
Pluripotente Stammzellen & Stammzellen der frühen Entwicklungsphase
Pluripotente Stammzellen & Stammzellen der frühen Entwicklungsphase
Anti-SSEA-1 Antibody, clone MC-480, PE conjugate is an antibody against SSEA-1 for use in IH, IP, IF, FC.
Qualität
Evaluated by Flow Cytometry with mouse embryonic stem cells.
Zielbeschreibung
220 kDa Calculated
Physikalische Form
Purified mouse monoclonal IgM conjugated to PE fluorochrome in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Lagerung und Haltbarkeit
Maintain refrigerated at 2-8oC in undiluted aliquots for up to 6 months from date of receipt. Protect from light.
Hinweis zur Analyse
Control
Mouse embryonic Stem Cells
Mouse embryonic Stem Cells
Sonstige Hinweise
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
This antibody reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG).
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Suggest suitable dilaution of SSEA-1 for flow cutometry.
1 answer-
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