AP182R

Donkey Anti-Rabbit IgG Antibody, Rhodamine conjugate, Species Adsorbed

Chemicon^®, from donkey

• UNSPSC Code: 12352203
• NACRES: NA.46
• eCl@ss: 32160702
• Conjugate: TRITC conjugate, rhodamine conjugate
• Clone: polyclonal
• Application: IF
• Citations: 22

Eigenschaften

• biological source: donkey
• Quality Segment: 100
• conjugate: TRITC conjugate, rhodamine conjugate
• antibody form: F(ab′)₂ fragment of affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• species reactivity: rabbit
• manufacturer/tradename: Chemicon^®
• technique(s): immunofluorescence: suitable
• shipped in: wet ice
• target post-translational modification: unmodified

Beschreibung

General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Application

Donkey anti-Rabbit IgG Antibody, Rhodamine conjugate, Species Adsorbed is an antibody against Donkey Rabbit IgG for use in IF.

Research Category
Secondary & Control Antibodies

Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling

Suggested dilution for most applications: 1:50-1:200

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Rabbit IgG

WAVELENGTH:
Absorption peak = 550nm, Emission peak = 570 nm.

Physical form

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.05% sodium azide.

RECONSTITUTION:
Reconstitute with 500 μL of sterile distilled water.

RHODAMINE/PROTEIN:
Approximately 0.52 (A550/A280)

Preparation Note

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• hcodes: H412
• pcodes: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3