ABN898

Anti-BMP2

from rabbit, purified by affinity chromatography

• Synonym(e): Bone morphogenetic protein 2, BMP-2, BMP-2A, Bone morphogenetic protein 2A
• UNSPSC-Code: 12352203
• eCl@ss: 32160702

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Methode(n): immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI-Hinterlegungsnummer: NP_001191
• UniProt-Hinterlegungsnummer: P12643
• Versandbedingung: ambient
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... BMP2(650)

Beschreibung

Allgemeine Beschreibung

Bone morphogenetic protein 2 (UniProt: P12643; also known as BMP-2, BMP-2A, Bone morphogenetic protein 2A) is encoded by the BMP2 (also known as BMP2A) gene (Gene ID 650) in human. BMP2 is a member of the transforming growth factor-beta (TGF-beta) superfamily. It is synthesized as a 60-kDa precursor that is processed in the secretory pathway to a small 18-kDa monomer; 2 monomers then associate to form the active homodimer, which binds to its receptor. BMP2 can induce bone formation and regeneration during early embryonic development. It is also involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. BMP2 is known to induce cartilage and bone formation and stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A, which leads to increased expression of ATF4 that plays a critical role in osteoblast differentiation.

Spezifität

This rabbit polyclonal antibody targets a region present in Prepro-, pro-, and mature human BMP2.

Immunogen

GST-tagged recombinant protein composed of the propeptide C-terminal region and the mature human BMP2 N-terminal region.

Anwendung

Research Category
Neurowissenschaft

Anti-BMP2, Cat. No. ABN898, is a highly specific rabbit polyclonal antibody that targets bone morphogenetic protein 2 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected BMP2 immunoreactivity in human prostate cancer, cerebellum, and tonsil tissue sections.

Qualität

Evaluated by Western Blotting in human kidney tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected BMP2 in 10 µg of human kidney tissue lysate.

Zielbeschreibung

~45 kDa observed. 44.70/42.48/12.90 kDa (Prepro-/pro-/mature human BMP2 monomer). Target band size appears larger due to glycosylation and dimerization. Uncharacterized bands may be observed in some lysate(s).

Verlinkung

Replaces: ABN303

Physikalische Form

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable