ABE1311

Anti-SETD5

from rabbit, purified by affinity chromatography

• Synonym(e): SET domain-containing protein 5
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Speziesreaktivität (Voraussage durch Homologie): rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
• Methode(n): immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI-Hinterlegungsnummer: NP_001073986.1
• UniProt-Hinterlegungsnummer: Q9C0A6
• Versandbedingung: ambient
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... SETD5(55209)

Beschreibung

Allgemeine Beschreibung

SET domain-containing protein 5 (UniProt: Q9C0A6; also known as Setd5) is encoded by the SETD5 (also known as KIAA1757) gene (Gene ID: 55209) in human. Setd5 protein is shown to be essential for mammalian development and is required for regulating germ cell-associated genes in embryonic stem cells. It interacts with Tbl1xr1 and Ctr9, which are components of 2 different transcriptional regulatory complexes (NcoR1 corepressor complex and Paf1 complex) in murine embryonic stem cells. Setd5-deficient embryonic stem cells display impaired cellular proliferation, increased apoptosis, defective cell cycle progression, a diminished ability to differentiate into cardiomyocytes, and greatly perturbed gene expression. Setd5-deficient mouse embryos exhibit severe defects in neural tube formation, somitogenesis and cardiac development, have aberrant vasculogenesis in embryos, yolk sacs, and placentas. They die between embryonic day 10.5 and 11.5. Three isoforms of Setd5 have been reported that are produced by alternative splicing. Its SET domain is localized to amino acids 269-390. Mutation in SETD5 gene has been linked to intellectual disability. (Ref.: Yu, SE., et al. (2017). Cell. Biochem. Funct. 35(5); 247-253; Osipovich, AB., et al. (2016). Development 143(24); 4595-4607).

Spezifität

This rabbit polyclonal antibody detects SET domain containing protein 5 in human cells. It targets an epitope within 19 amino acids from the N-terminal region.

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of human SET domain-containing protein 5.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Anti-SETD5, Cat. No. ABE1311, is a highly specific rabbit polyclonal antibody that targets SET domain-containing protein 5 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected SETD5 in human cerebral cortex, human pancreas, and human testis tissue sections.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected SETD5 in U2OS cells.

Qualität

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SETD5 in 10 µg of HeLa nuclear extract.

Zielbeschreibung

~150 kDa observed; 158.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physikalische Form

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1