AB5884

Anti-HCN1 Antibody

Chemicon^®, from rabbit

• Synonym(e): Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 1, HAC2, BCNG1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC, WB
• Citations: 12

Eigenschaften

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable, western blot: suitable
• NCBI accession no.: NM_021072.2
• UniProt accession no.: O60741
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... HCN1(348980)

Beschreibung

Immunogen

Synthetic peptide corresponding amino acids 6-24 of rat HCN-1 (Accession number AAF62173).

Application

Anti-HCN1 Antibody is an antibody against HCN1 for use in IH & WB.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Western blot: 1:200-1:400 using ECL on rat brain membranes.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes HCN-1 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 1, HAC2, BCNG1). The epitope does not share homology with any other known proteins.

SPECIES REACTIVITIES: It is expected that the antibody will also react with mouse (18/19) and possibly rabbit (16/19) due to sequence homology. Other species have not been tested.

Physical form

Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Preparation Note

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• hcodes: H412
• pcodes: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3