AB125
Anti-Serotonin Antibody
serum, Chemicon®
Synonym(e):
5-HT
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Über diesen Artikel
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
rat
Application:
IHC
Citations:
14
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
shipped in
dry ice
target post-translational modification
unmodified
Immunogen
5-Hydroxytryptamine-glutaraldehyde-Poly-lysine
Application
Anti-Serotonin Antibody detects level of Serotonin & has been published & validated for use in IH.
Immunohistochemistry: 1:1,000-1:2,500 by ABC or enhanced detection. Glutaraldehyde fixation is required. Antibody will not react with non-glutaraldehyde treated tissues. Blocking should be with serum or BSA containing 0.1-0.2% triton X-100; remove all blocking an detergents during primary antibody incubation. Dilute only in PBS or TBS for best results. For fluorescent detection antibody dilution must be reduced, but it is not recommended because of glutaraldehyde autofluorescence.
Optimal working dilutions must be determined by the end user.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
Biochem/physiol Actions
Serotonin (5-Hydroxytryptamine)
The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity
5-Hydroxytryptamine-G-PL 1
5-Methoxytryptamine-G-PL 1/25
Tryptamine-G-PL 1/60
5-Hydroxytryptophan-G-PL 1/175
5-Hydroxytryptamine 1/1150
Tryptophan-G-PL 1/3400
5-Methoxytryptophan-G-PL 1/19800
The antisera was also tested for specificity using the free-floating PAP technique on rat
raphe nuclei.
Abbreviations:
(G) Glutaraldehyde
(=) Non-reduced conjugate
(PL) Poly-lysine
The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity
5-Hydroxytryptamine-G-PL 1
5-Methoxytryptamine-G-PL 1/25
Tryptamine-G-PL 1/60
5-Hydroxytryptophan-G-PL 1/175
5-Hydroxytryptamine 1/1150
Tryptophan-G-PL 1/3400
5-Methoxytryptophan-G-PL 1/19800
The antisera was also tested for specificity using the free-floating PAP technique on rat
raphe nuclei.
Abbreviations:
(G) Glutaraldehyde
(=) Non-reduced conjugate
(PL) Poly-lysine
Physical form
Rabbit antiserum. Liquid with 0.05% sodium azide.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk
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