71754-M

Terrific Broth - Novagen

Terrific Broth is a highly enriched granulated medium to improve the yield of plasmid DNA from E. coli.

• Synonym(e): TB media
• NACRES: NA.54
• UNSPSC Code: 41106213

Eigenschaften

• Quality Segment: 200
• form: granular
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze
• technique(s): microbiological culture: suitable
• application(s): microbiology
• storage temp.: 10-30°C

Beschreibung

General description

Terrific Broth (TB) is one of the complex media that is widely used to grow Escherichia coli in small-scale cultures. [1] Terrific Broth is a highly enriched granulated medium to improve the yield of plasmid DNA from E. coli. The granules ensure rapid and uniform dissolution in water, and prevent clumping of the medium and inhalation of the airborne powder. The composition of the Terrific Broth per liter is 12 g tryptone, 24 g yeast extract, 9.4 g potassium phosphate, dibasic, and 2.2 g potassium phosphate, monobasic. Use 47.6 g of granulated medium per liter of water.

Application

Terrific Broth – Novagen has been used to culture BLR(DE3) bacteria for protein polymer synthesis[2] and production of recombinant antigens for autoantibody assays[3]

Features and Benefits

• Media preparation is simple and quick
• ready to use EasyPak media packets: Just add water and sterilize
• Consistent, high-quality media for E.coli cells

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3