539508

PP2A₁, Bovine Kidney

• Synonym(e): Protein Phosphatase 2A₁
• UNSPSC Code: 12352202
• EG-Nummer: 3.1.3.16 (BRENDA, IUBMB)
• Form: liquid
• Assay: ≥90% (SDS-PAGE)

Eigenschaften

• assay: ≥90% (SDS-PAGE)
• Quality Segment: 100
• form: liquid
• specific activity: ≥750 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• shipped in: wet ice
• storage temp.: −20°C

Beschreibung

General description

Native, PP2A₁ from bovine kidney. A divalent cation-independent protein serine/threonine phosphatase involved in the control of numerous cellular processes. Has a catalytic subunit of 36 kDa, a subunit of 55 kDa, and a regulatory subunit of 60 kDa. Reported to associate with microtubules and to bind to purified tubulin polymerized by TAXOL^® Drug.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM Tris-HCl, 14 mM β-ME, 1 mM benzamidine, 1 mM EDTA, 100 µM PMSF, 50% glycerol, pH 7.0.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Hiraga, A. and Tamura, S. 2000. Biochem. J.346, 433.
Ferrigno, P., et al. 1993. Mol. Biol. Cell. 4, 669.
Guo, H. and Damuni, Z. 1993. Proc. Natl. Acad. Sci. USA90, 2500.
Hendrix, P., et al. 1993. J. Biol. Chem.268, 15267.
Amick, G.D., et al. 1992. Biochem. J. 287, 1019.
Cohen, P., et al. 1990. Trends Biochem. Sci.15, 98.
Damuni, Z., et al. 1989. J. Biol. Chem.264, 6412.1

One unit is defined as the amount of enzyme that will release 1.0 nmol of [³²P]P_i from ³²P-labeled phosphorylase per min at 30°C, pH 7.0.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Taxol is a registered trademark of Bristol-Myers Squibb Co.

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable