524736

Phosphodiesterase 4B2, His•Tag^®, Human, Recombinant, S. frugiperda

• Synonym(e): PDE4B2, cAMP-Specific Phosphodiesterase 4B2
• NACRES: NA.54
• UNSPSC Code: 12352202
• Specific activity: ≥0.25 U/μL
• Recombinant: expressed in baculovirus infected Spodoptera frugiperda cells

Eigenschaften

• recombinant: expressed in baculovirus infected Spodoptera frugiperda cells
• Quality Segment: 100
• form: liquid
• specific activity: ≥0.25 U/μL
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Beschreibung

General description

Full-length, recombinant, human phosphodiesterase 4B2 (accession no. L20971) fused at the N-terminus to a His•Tag sequence and expressed in S. frugiperda using a baculovirus expression system. Plays a significant role in the regulation of inflammatory cell activation. PDE4B2 is a predominant isoform in neutrophils and monocytes. Supplied as a partially purified, catalytically active preparation.

Physical form

In 150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol.538, 39.
Smith, P.G., et al. 2005 Blood105, 308.
Baroja, M.L., et al. 1999. J. Immunol.162, 2016.

One unit is defined as the amount of enzyme that will convert 1 pmol cAMP to AMP per min at 30°C, pH 7.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: WGK 1
• Signalwort: Danger
• Gefahrencodes: EUH430
• Sicherheitshinweise: P201 - P202 - P273 - P391 - P405 - P501
• Hazard Classifications: ED ENV 1