516852

Pertussis Toxin, B Oligomer, Bordetella pertussis - Calbiochem

Eigenschaften

• form: lyophilized
• impurities: ≤1% holotoxin (by CHO cell assay)
• solubility: aqueous buffer: soluble, sterile distilled water: soluble

Beschreibung

General description

The pentameric cell-binding component that is responsible for binding of the holotoxin to eukaryotic cell surfaces, facilitating entry of the A protomer into receptive cells. Reported to elicit direct cellular responses by binding to several cell surface receptors with oligosaccharide side chains. Also elicits a variety of physiological responses, such as mitogenesis in human T cells, enhancement of aggregation of human platelets, elevation of cytosolic Ca²⁺ levels, and neutralization of antibody response in mice. Reported to deactivate CCR5 and inhibit the entry of R5 HIV-1 in activated T lymphocytes. Also blocks post-entry step of HIV-1 replication.

Biochem/physiol Actions

Cell surface receptors with oligosaccharide side chains

Preparation Note

Following reconstitution, refrigerate (4°C). Stock solutions, prepared from high ionic strength buffers, are stable for up to 3 months at 4°C.

Analysis Note

Four distinct bands by SDS-PAGE