G 418 Sulfate, Sterile-Filtered Solution in PBS, Cell Culture-Tested

Name: G 418 Sulfate, Sterile-Filtered Solution in PBS, Cell Culture-Tested
Brand: MM

Synonym(e):

G 418 Sulfate, Sterile-Filtered Solution in PBS, Cell Culture-Tested, G418, G 418

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Über diesen Artikel

Empirische Formel (Hill-System):

C₂₀H₄₀N₄O₁₀ · 2H₂SO₄

CAS-Nummer:

108321-42-2

Molekulargewicht:

692.71

UNSPSC Code:

12352203

MDL number:

MFCD00058314

Technischer Dienst

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Unterstützung erhalten

Eigenschaften

assay

≥98% (TLC)

Quality Segment

200

form

liquid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles, protect from light

storage temp.

2-8°C

InChI

1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1

InChI key

UHEPSJJJMTWUCP-NKCAIAFTSA-N

Beschreibung

General description

The optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 µg/ml Mammalian cells: 0.1-2.0 µg/ml

Packaging

Packaged under inert gas

Physical form

Sterile-filtered solution of Cat. No. 345810 supplied at 50 mg/ml in PBS, pH 7.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 1 year at -20°C.

Other Notes

Ethier, S.P., and Taback, E. 1993. Cancer Lett.74, 189.
Santerre, R.F., et al. 1991. Methods Mol. Biol.7, 245.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol.133, 46.
Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol.163, 8.
Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA79, 5166.
Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA79, 7356.
Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun.101, 1031.
Jimenez, A., and Davies, J. 1980. Nature287, 869.