382180

Histone Deacetylase 6, GST-Fusion, Human Recombinant, S. frugiperda

Histone Deacetylase 6, GST-Fusion, Human Recombinant, S. frugiperda, is a full-length HDAC6 fused to GST at the N-terminus. Useful in the study of HDAC6 regulation and for inhibitor screening.

• Synonym(e): HDAC6, Human, Recombinant
• NACRES: NA.54
• UNSPSC Code: 12352202
• Specific activity: ≥50 units/μg protein
• Assay: ≥80% (SDS-PAGE)
• Recombinant: expressed in insect cells (S. frugiperda)

Eigenschaften

• recombinant: expressed in insect cells (S. frugiperda)
• Quality Level: 100
• assay: ≥80% (SDS-PAGE)
• form: liquid
• specific activity: ≥50 units/μg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Beschreibung

General description

Full length, recombinant, human histone deacetylase 6 (HDAC6) fused to GST at the N-terminus and expressed in S. frugiperda insect cells using a baculovirus expression system. Actively maintained in the cytoplasm, HDAC6 is uniquely dependent on its ubiquitin-binding domain and functions at the nexus between two cellular signaling systems, involving protein acetylation and ubiquitination. The active deacetylase is useful in the study of HDAC6 regulation and for inhibitor screening.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

40 mM Tris-HCl pH8.0, 110mM NaCl, 2.2 mM KCl, 0.04% Tween 20, 3mM DTT, and 20% glycerol.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

One unit is defined as the amount of enyme that is required to deacetylate 1 pmol fluorescent HDAC peptide substrate per min at 30°C.

Pandey, U.B., et al. 2007. Nature447, 859.
Hubbert, C., et al. 2002. Nature417, 455.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1