317639

Dipeptidylpeptidase IV, His•Tag^®, Human, Recombinant, S. frugiperda

DPPIV is involved in many cellular processes such as activation of cytokines, differentiation, and cell-matrix interactions. Inhibition of DPPIV has been reported to be an effective treatment for type II diabetes.

• EG-Nummer: 3.4.14.5 (BRENDA, IUBMB)
• NACRES: NA.54
• UNSPSC Code: 12352202
• Specific activity: ≥8 units/mg protein
• Assay: ≥90% (SDS-PAGE)

Eigenschaften

• Quality Segment: 100
• assay: ≥90% (SDS-PAGE)
• form: liquid
• specific activity: ≥8 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Beschreibung

General description

DPPIV is involved in many cellular processes such as activation of cytokines, differentiation, and cell-matrix interactions. Inhibition of DPPIV has been reported to be an effective treatment for type II diabetes.

Note: 1 mU = 1 milliunit.

Recombinant, human DPP IV fused at the N-terminus to a His•Tag sequence and expressed in S. frugiperda insect cells. A serine exopeptidase dimer composed of two identical subunits of 110-130 kDa. DPPIV is involved in many cellular processes such as activation of cytokines, differentiation, and cell-matrix interactions. Inhibition of DPPIV has been reported to be an effective treatment for type II diabetes. Note: 1 mU = 1 milliunit.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 20 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% NaN₃, pH 8.0.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

One unit is defined as the amount of enzyme that will hydrolyze 1 µmole H-Gly-Pro-AMC per min at 37°C, pH 8.0.

Pospisilik, JA, et al. 2003. Diabetes52, 741.
Dobers, J., et al. 2002. Protein Expr. Purif.25, 527.
Marguet, D., et al. 2000. Proc. Natl. Acad. Sci.97, 6874.
Misumi, Y., et al. 1992. Biochim. Biophys. Acta1131, 333.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• wgk: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash