251765

DAR-4M AM

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.

• Synonym(e): DAR-4M AM, Diaminorhodamine-4M AM
• Empirische Formel (Hill-System): C₂₈H₃₁IN₄O₅
• Molekulargewicht: 630.47
• NACRES: NA.25
• UNSPSC Code: 12352202

Eigenschaften

• Quality Segment: 100
• assay: ≥98% (HPLC)
• form: solution
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, protect from light
• color: dark red
• fluorescence: λ_ex ~560 nm, λ_em ~575 nm
• shipped in: ambient
• storage temp.: −20°C

Beschreibung

General description

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760) by the action of esterases. Reacts with NO, in the presence of O₂, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Nitric oxide (NO) fluorescent indicator

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Physical form

In DMSO.

Preparation Note

Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution, discard any unused portion.

Other Notes

Kojima, H., et al. 2001. Anal. Chem.73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.

Legal Information

Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: 188.6 °F
• flash_point_c: 87 °C