208912

Anti-Calreticulin Mouse mAb (FMC75)

liquid, clone FMC75, Calbiochem^®

• NACRES: NA.43
• UNSPSC Code: 12352203
• Clone: FMC75, monoclonal
• Species reactivity: human, monkey
• Application: —
• Citations: 3

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: FMC75, monoclonal
• form: liquid
• does not contain: preservative
• species reactivity: human, monkey
• should not react with: rat, E. coli, canine, hamster, salmon, bovine, mouse, guinea pig
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• dilution: (Enzyme Immunoassay (0.1 µg/mL); Immunoblotting (0.5 µg/mL); Immunocytochemistry ; Immunohistochemistry ; Flow Cytometry )
• isotype: IgG1
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Beschreibung

General description

Anti-Calreticulin, mouse monoclonal, clone FMC75, recognizes the ~63 kDa calreticulin in Molt 4, MCF-7, HeLa, K562, A431 & other cells. It is validated for FC, WB, ICC, IHC, and in enzyme immunoassay.

Protein G purified mouse monoclonal antibody. Recognizes the ~63 kDa (apparent MW) calreticulin protein.

Recognizes the ~63 kDa (apparent MW) calreticulin protein in Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198, and vero cells.

Immunogen

Human

a recombinant protein consisting of human calreticulin fused to maltose binding protein

Application

Enzyme Immunoassay (0.1 µg/ml)

Immunoblotting (0.5 µg/ml, colorimetric; see application references)

Immunocytochemistry (see application references)

Immunohistochemistry (see application references)

Flow Cytometry (see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 50% Glycerol and 0.09% Sodium Azide.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Human Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198 cells, or monkey Vero cells

Other Notes

Does not cross-react with GrpE or Grp78. Variables associated with assay conditions will dictate the proper working dilution.

Nauseef, W.M., et al. 1995. J. Biol. Chem. 270, 4741.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J.8, 3581.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable