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BMS

Water-soluble reagent useful for the reduction of native disulfide bonds in proteins. Considered to be a superior reducing agent to DTT.

• Synonym(e): BMS, bis(2-Mercaptoethylsulfone)
• Empirische Formel (Hill-System): C₄H₁₀O₂S₃
• CAS-Nummer: 145626-87-5
• Molekulargewicht: 186.32
• UNSPSC-Code: 12352128

Eigenschaften

• Qualitätsniveau: 100
• Assay: ≥99% (titration)
• Form: crystalline solid
• Eignung der Reaktion: reagent type: reductant
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Farbe: white
• pKa: (1) 7.9, (2) 9.0
• Versandbedingung: ambient
• Lagertemp.: −20°C
• InChI: 1S/C4H10O2S3/c5-9(6,3-1-7)4-2-8/h7-8H,1-4H2
• InChIKey: YWXCBLUCVBSYNJ-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Water-soluble reagent useful for the reduction of native disulfide bonds in proteins. Considered to be a superior reducing agent to DTT. Shown to reduce accessible disulfide bonds in immunoglobulin and trypsinogen under non-denaturing conditions 5 to 7 times faster than DTT at pH 7. The relatively less accessible disulfide bond in α-chymotrypsinogen A was reduced 2.3 times faster at pH 7 with BMS than with DTT. Has a pK_a of 7.9 and 9.0.

Water-soluble reagent useful for the reduction of native disulfide bonds in proteins. Considered to be a superior reducing agent to DTT. Shown to reduce accessible disulfide bonds in immunoglobulin and trypsinogen under non-denaturing conditions 5 to 7 times faster than DTT at pH 7. The relatively less accessible disulfide bond in α-chymotrypsinogen A was reduced 2.3 times faster at with BMS than with DTT at pH 7.0.

Verpackung

Packaged under inert gas

Warnhinweis

Toxicity: Standard Handling (A)

Angaben zur Herstellung

May require warming (40-45°C) to achieve complete solubilization in H₂O.

Sonstige Hinweise

Singh, R., et al. 1995. Methods Enzymol.251, 167.
Singh, R., and Whitesides, G.M. 1994. Bioorg. Chem.22, 109.
Singh, R., and Whitesides, G.M. 1994. Protein Sci. 3, Suppl. 1, 430T.
Lamoureux, G.V., and Whitesides, G.M. 1993. J. Org. Chem.58, 633.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable