203325

BMS

Water-soluble reagent useful for the reduction of native disulfide bonds in proteins. Considered to be a superior reducing agent to DTT.

• Synonym(e): BMS, bis(2-Mercaptoethylsulfone)
• Empirische Formel (Hill-System): C₄H₁₀O₂S₃
• CAS-Nummer: 145626-87-5
• Molekulargewicht: 186.32
• MDL number: MFCD00467637
• UNSPSC Code: 12352128

Eigenschaften

• Quality Segment: 100
• assay: ≥99% (titration)
• form: crystalline solid
• reaction suitability: reagent type: reductant
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• pKa: (1) 7.9, (2) 9.0
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: [S](=O)(=O)(CCS)CCS
• InChI: 1S/C4H10O2S3/c5-9(6,3-1-7)4-2-8/h7-8H,1-4H2
• InChI key: YWXCBLUCVBSYNJ-UHFFFAOYSA-N

Beschreibung

General description

Water-soluble reagent useful for the reduction of native disulfide bonds in proteins. Considered to be a superior reducing agent to DTT. Shown to reduce accessible disulfide bonds in immunoglobulin and trypsinogen under non-denaturing conditions 5 to 7 times faster than DTT at pH 7. The relatively less accessible disulfide bond in α-chymotrypsinogen A was reduced 2.3 times faster at pH 7 with BMS than with DTT. Has a pK_a of 7.9 and 9.0.

Packaging

Packaged under inert gas

Preparation Note

May require warming (40-45°C) to achieve complete solubilization in H₂O.

Other Notes

Singh, R., et al. 1995. Methods Enzymol.251, 167.
Singh, R., and Whitesides, G.M. 1994. Bioorg. Chem.22, 109.
Singh, R., and Whitesides, G.M. 1994. Protein Sci. 3, Suppl. 1, 430T.
Lamoureux, G.V., and Whitesides, G.M. 1993. J. Org. Chem.58, 633.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable