15-105

mTOR phosphorylation Pathway Explorer Antibody MiniPack

This Antibody pack of Anti-mTOR antibodies contains Anti-phospho-mTOR Antibody(Ser2481), Anti-phospho-mTOR Antibody (Thr2446), Anti-phospho-mTOR (Ser2448).

• Synonym(e): FKBP12-rapamycin complex-associated protein 1, FKBP-rapamycin associated protein, Mammalian target of rapamycin, Rapamycin target protein, rapamycin associated protein FRAP2
• UNSPSC Code: 12161503
• NACRES: NA.77
• eCl@ss: 32161000
• Conjugate: unconjugated
• Clone: polyclonal
• Application: activity assay
• Citations: 4

Eigenschaften

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• clone: polyclonal
• purified by: affinity chromatography
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable
• NCBI accession no.: NP_004949
• UniProt accession no.: P42345
• shipped in: dry ice
• target post-translational modification: phosphorylation (pSer2481/pSer2446/pSer2448)
• Gene Information: human ... MTOR(2475)

Beschreibung

General description

Pathway Explorer Antibody MiniPack:
Each Pathway Explorer Antibody Minipack contains three related antibodies as part of a signaling cascade or a combination of total and phosphorylated forms of key signaling targets. Each of the three antibodies are 30% the original pack size. Full size versions of each of the Pathway Explorer antibodies are available for sale individually under the same catalog number with the removal of “SP” off of each one (e.g. 05-591SP can be ordered as 05-591).

mTOR:
mTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. Interestingly, mTOR is the central component of two multimeric kinase complexes consisting of mTOR and numerous other mTOR binding proteins. These two multimeric protein complexes are designated mTORC1 and mTORC2. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and GβL (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. The complex is activated primarily though the PI3 Kinase/Akt pathway. Upon insulin stimulation, Akt activates mTORC1 by phosphorylating and inhibiting TSC (and possibly other yet discovered targets and/or mTOR itself). This inhibits the upstream small GTPase regulator Rheb (Ras homolog enriched in brain). This inhibits the kinase activity of the mTORC1 complex, thus disabling its ability to phosphorylate its downstream targets such as p70 S6K on Thr389 and 4E-BP1 on Thr229. The other mTOR complex, mTORC2 (mTOR Complex 2), is made up of at least mTOR, Rictor, GL, Sin1, Protor 1 and 2. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Interestingly, unlike mTORC1, the mTORC2 complex appears to not be inhibited by treatment with rapamycin and for this reason is referred to as the rapamycin-insensitive complex. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.

*See full size versions for corresponding references.

Immunogen

Epitope: 09-345SP:phosphorylated Thr2446 of mTOR
09-213SP: phosphorylated Ser2448 of mTOR
09-343SP: phosphorylated Ser2481 of mTOR

Application

Pathway Explorer Antibody MiniPack

Research Category
Signaling

Apoptosis & Cancer

Research Sub Category
PI3K, Akt, & mTOR Signaling

Glucose/Glycogen Metabolism

Insulin/Energy Signaling

This Antibody pack of Anti-mTOR antibodies contains Anti-phospho-mTOR Antibody(Ser2481), Anti-phospho-mTOR Antibody (Thr2446), Anti-phospho-mTOR (Ser2448).

Packaging

Each vial is 30% the size of the parent catalog number

Physical form

3 individual tubes each containing antibodies directed against one of the three phosphorylated forms of mTOR (Thr2446, Ser2448, and Ser2481)

Other Notes

09-343SP Anti-phospho-mTOR (Ser2481); 30 µL

09-345SP Anti-phospho-mTOR (Thr2446); 30 µL

09-213SP Anti-phospho-mTOR (Ser2448); 60 µL

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids