14-779-M

MKP5, active

• UNSPSC Code: 12352204
• NACRES: NA.32
• eCl@ss: 32160405
• Biological source: human

Eigenschaften

• biological source: human
• Quality Segment: 100
• mol wt: M_w 20.6 kDa
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (phosphatase)
• NCBI accession no.: NM_007207

Beschreibung

General description

Dual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the
phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAPK superfamily
(MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members ofthis family of dual specificity phosphatases show distinct substrate specificities for MAPKs, different tissue
distribution and subcellular localization, and different modes of inducibility of their expression by extracellular
stimuli. This gene product binds to and inactivates p38 and SAPK/JNK, but not MAPK/ERK. Its subcellular localization
is unique; it is evenly distributed in both the cytoplasm and the nucleus. This gene is widely expressed in various
tissues and organs, and its expression is elevated by stress stimuli. Three transcript variants encoding two different
isoforms have been found for this gene.

N-terminal 6His-tagged, recombinant, human MKP5, amino acids 320-end, expressed in E. coli.

Packaging

Also available in
250μg
size as catalog #
14-779M
and in
bulk
as catalog #
14-779-K
.

Physical form

Protein in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Format: Purified using Ni2+/NTA agarose.

Preparation Note

6 months at -70°C

Analysis Note

Routinely evaluated by the release of phosphate from the substrate 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP)

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• Gefahrencodes: H317
• Sicherheitshinweise: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable