07-1458

Anti-ROCK-1/2 Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): Renal carcinoma antigen NY-REN-35, Rho kinase, Rho-associated, coiled-coil containing protein kinase 1, Rho-associated, coiled-coil-containing protein kinase 1, p160 ROCK-1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: human, mouse
• Application: WB
• Citations: 12

Eigenschaften

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, mouse
• technique(s): western blot: suitable
• NCBI accession no.: NP_005397.1
• UniProt accession no.: Q13464
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ROCK1(6093) ; mouse ... Rock1(19877)

Beschreibung

General description

158 kDa

Rho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROKα/ROCK-II and ROKβ/ROCK-I. These large cellular proteins (150-160 kDa), have been reported to be involved in the formation of stress fibers, focal adhesions and cardiac hypertonic responses. Current research suggests that ROK of smooth muscle may be involved in the contractile process via phosphorylation of MYPT1 and Myosin. The serine-threonine kinase ROCK-1 is a down-stream effector of Rho, and is involved in signal transduction pathways that regulate cell morphology, motility, and tumor progression through the reorganization of the actin cytoskeleton.

Immunogen

Synthetic peptide corresponding to human ROCK-1, 90.5% homology with ROCK-2

Application

This Anti-ROCK-1/2 Antibody is validated for use in WB for the detection of ROCK-1/2.

Biochem/physiol Actions

This antibody recognizes ROCK-1 and ROCK-2.

Analysis Note

Evaluated by Western Blot in A431 cell lysate.
Western Blot Analysis: 1:500 dilution of this antibody detected ROCK-1/2 on 10 µg of A431 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-1121

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable