06-1334

Anti-phospho-TAB1 (Thr431) Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): TGF-beta activated kinase 1/MAP3K7 binding protein 1, TAK1-binding protein 1, transforming growth factor beta-activated kinase-binding protein 1, mitogen-activated protein kinase kinase kinase 7 interacting protein 1, Mitogen-activated protein kinase kin
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: rat, human, horse, mouse
• Application: WB
• Citations: 4

Eigenschaften

• biological source: rabbit
• Quality Segment: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat, human, horse, mouse
• species reactivity (predicted by homology): equine (based on 100% sequence homology), ox (based on 100% sequence homology)
• technique(s): western blot: suitable
• NCBI accession no.: NP_006107
• UniProt accession no.: Q15750
• shipped in: wet ice
• target post-translational modification: phosphorylation (pThr431)
• Gene Information: human ... TAB1(10454)

Beschreibung

General description

TAK1 binding protein 1 (TAB1) is thought to activate TAK1 MAPKKK in transforming growth factor-beta (TGF-beta) signaling. TAB1 can also associate with TAK1 to form a unique MAPKKK complex that is thought to activate AP-1 and the NFkappaB signaling pathways. Through conformational changes, TAB1 is also thought to induce p38alpha autophosphorylation. It has been linked to both IL-1alpha and TNFalpha signaling and therefore may also be involved in the inflammatory response. Recent research has found that the TAK1-TAB1 complex may have anti-inflammatory effects by aiding stercurensin in regulating the NFκB-dependent inflammatory pathways.

~78 kDa observed. 55 kDa calculated. A higher molecular weight on western blot may be due to glycosylation. An uncharacterized band appears at ~42 and 50 kDa in some lysates.

Immunogen

Epitope: Phosphorylated Thr431

KLH-conjugated linear peptide corresponding to human TAB1 phosphorylated at Thr431.

Application

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This Anti-phospho-TAB1 (Thr431) Antibody is validated for use in WB for the detection of phospho-TAB1 (Thr431).

Biochem/physiol Actions

This antibody recognizes TAB1 phosphorylated at Thr431.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
L6 cell lysate

Evaluated by Western Blot in L6 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected TAB1 on 10 µg of L6 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1