05-172-AF488

Anti-IGF-I Antibody, clone Sm1.2, Alexa Fluor^™ 488 Conjugate

clone Sm1.2, from mouse, ALEXA FLUOR^™ 488

• Synonym(e): Insulin-like growth factor I, IGF-I, Mechano growth factor, MGF, Somatomedin-C
• UNSPSC Code: 12352203
• NACRES: NA.45
• eCl@ss: 32160702
• Conjugate: ALEXA FLUOR^™ 488
• Clone: Sm1.2, monoclonal
• Application: ICC
• Citations: -

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• conjugate: ALEXA FLUOR^™ 488
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: Sm1.2, monoclonal
• species reactivity: chicken, rat, mouse, human, fish
• technique(s): immunocytochemistry: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_000609
• UniProt accession no.: P05019
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... IGF1(3479)

Beschreibung

General description

Insulin-like growth factor I (UniProt P05019; also known as IGF-I, Somatomedin-C) is encoded by the IGF1 (also known as IGFI, MGF) gene (Gene ID 3479) in human. Human insulin-like growth factor-I (IGF-I) is a single chain 70 amino acid polypeptide that shares structural homology with insulin and human IGF-II. IGF-I plays a critical role in growth, differentiation and development via autocrine, paracrine and endocrine mechanisms. It is a mitogen and also has effects on cell migration and differentiation. IGF-I is a regulator of metabolism and is also implicated in the pathophysiology of cancer, diabetes and obesity. Upon binding the tetrameric (alpha:beta)2 IGF-I receptor, the receptor alpha chains induce the tyrosine autophosphorylation of the beta chains, which in turn trigger cell type-specific downstream signaling cascade of events.

~17 kDa observed

Immunogen

Highly purified human plasma insulin-like growth factor I (IGF-I; Somatomedin C; Sm-C) conjugated to mouse albumin (Van Wyk, J.J., and Bruton, E.T. (1997). Endocrinology. 138(10):4521-4522). Action of somatomedins on cell growth: Effect of selective neutralisation of somatomedin C (IGF-I) with a monoclonal antibody. In: Raiti, S., Tolman, and R.A. (eds.), Human Growth Hormone. pp. 585–599. Plenum Publishing Corp., New York, NY).

Application

Anti-IGF-I Antibody, clone Sm1.2, Alexa Fluor^™ 488 Conjugate is an antibody against IGF-I for use in Immunocytochemistry.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected IGF-I in HeLa and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-172) is shown to be suitable also for Western blotting, immunohistochemistry, immunoprecipitation, and neutralization applications.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Biochem/physiol Actions

IGF-I (Russel, W.E., (1984). Proc. Natl. Acad. Sci. U. S. A. 81(8):2389-2392). 40% cross reactivity to IGF-II (Van Wyk, J.J., and Bruton, E.T. (1997). Endocrinology. 138(10):4521-4522).

Physical form

Protein G Purified

Purified mouse antibody conjugate in PBS with 15mg/mL BSA and 0.1 % sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected IGF-I in A431 cells.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: WGK 2