05-1355

Anti-acetyl Histone H4 (Lys5/8/12/16) Antibody, clone 3HH4-4C10

ascites fluid, clone 3HH4-4C10, from mouse

• Synonym(e): H4KAc, Histone H4 (acetyl), H4 histone family, member N, H4 histone, family 2, histone 2, H4, histone 2, H4a, histone IV, family 2, histone cluster 2, H4a
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: 3HH4-4C10, monoclonal
• Species reactivity: human
• Application: ICC, WB
• Citations: 12

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 3HH4-4C10, monoclonal
• species reactivity: human
• species reactivity (predicted by homology): mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable, western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_778224
• UniProt accession no.: P62805
• shipped in: dry ice
• target post-translational modification: acetylation (Lys5/Lys8/Lys12/Lys16)
• Gene Information: human ... H4C1(8359) ; mouse ... H4C1(326619)

Beschreibung

General description

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Posttranslational acetylation of the conserved core histone N-terminal tail domains is typically associated with gene activation.

~ 13 kDa

Immunogen

Epitope: Lys5/8/12/16

Linear peptide corresponding to human Histone H4 acetylated at Lys5/8/12/16.

Application

Immunocytochemistry Analysis:
1:500 dilution from a representative lot detected Histone H4 in A431 and HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Use Anti-acetyl Histone H4 (Lys5/8/12/16) Antibody, clone 3HH4-4C10 (mouse monoclonal antibody) validated in WB, ICC to detect acetyl Histone H4 (Lys5/8/12/16) also known as H4KAc, Histone H4 (acetyl).

Biochem/physiol Actions

This antibody recognizes Histone H4.

Physical form

Unpurified

Unpurified mouse monoclonal IgG1κ preservative-free ascites.

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HeLa acid extract lysate

Evaluated by Western Blot in HeLa acid extract lysate.

Western Blot Analysis: 1:2,000 dilution of this antibody detected Histone H4 on 10 µg of HeLa acid extract lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: WGK 1